, Volume 52, Issue 9–10, pp 579–583 | Cite as

Validation of an isocratic HPLC method based on the use of ABZ+ plus phase for the simultaneous determination of methylxantines, chlorogenic acid, some hydroxy-benzoic and hydroxy-cinnamic acids. application to cocoa, coffee, tea and cola-drinks

  • M. Arlorio
  • J. D. Coïsson
  • A. Martelli
Originals Column Liquid Chromatography


Validation of an isocratic HPLC method for determination of methylxantines, chlorogenic acid and some hydroxy-benzoic/cinnamic acids in nervine foods and beverages is described. The method proposed can be usefully employed by single-wavelength/UV/HPLC-equipped Quality Control Laboratories. The ABZ+ Plus phase permitted complete separation of 10 compounds in 20 min. k, asymmetry of peaks, number of theoretical plates at different pH (3.0, 3.5, 4.0) and temperature (30, 40°C) were considered to determine best separation conditions. The method was then applied to some commercial samples of cocoa, coffee, tea and cola drinks, showing its real usefulness on quality control of these foods, particularly in monitoring roasting processes.

Key Words

Column liquid chromatography Hydroxy-cinnamic and hydroxy-benzoic acids Methylxantines Chlorogenic acid 


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  1. [1]
    Spiller, M.A. In:The methylxantine beverages and foods: chemistry, consumption and health effects. Alan Liss, New York,1984.Google Scholar
  2. [2]
    Purdon, M.P.; McCamey, D.A.J. Food Sci. 1987,52, 1680–1683.CrossRefGoogle Scholar
  3. [3]
    Trugo, L.C.; Macrae, R.Food Chem. 1984,15, 219–227.CrossRefGoogle Scholar
  4. [4]
    Pictet, G.; Rehacek, J. ASIC, 10e Colloque, Salvador,1982.Google Scholar
  5. [5]
    Amorim, H.V.; Teixeira, A.A.; Guercio, M.A.; Cruz, V.F.; Malavolta, E.Turrialba 1974,24, 217–221.Google Scholar
  6. [6]
    Osawa, K.; Matsumoto, T.; Maruyama, T.; Naito, Y.; Okuda, K.; Takazoe, I.Bull. Tokyo Dent. Coll. 1990,31, 125–128.Google Scholar
  7. [7]
    Azizah, A.H.; Ruslawati Nik, N.M.; Swee Tee, T.Food Chem. 1999, 64, 199–202.CrossRefGoogle Scholar
  8. [8]
    Shahrzad, S.; Bitsch, I.J. Chromatography A 1996,16, 223–231.CrossRefGoogle Scholar
  9. [9]
    Sanbongi, C.; Osakabe, N.; Netsume, M.; Takizawa, T.; Gomi, S.; Osawa, T.J. Agric. Food Chem. 1998,46, 454–457.CrossRefGoogle Scholar
  10. [10]
    Yen, G.C.; Duh, P.D.J. Am. Oil Chem. Soc. 1993,70, 383–386.CrossRefGoogle Scholar
  11. [11]
    Nakakuki, H.; Horie, H.; Yamauchi, Y.; Kohata, K.J. Chromatogr. A 1999,848, 523–527.CrossRefGoogle Scholar
  12. [12]
    Kouno, Y.; Samizo, K.; Ishizuka, H.; Ishikura, C.Yakugaku Zasshi 1997,117, 178–184.Google Scholar
  13. [13]
    Schreiber-Deturmeny, E.; Bruguerolle, B.J. Chromatogr. B Biomed. Appl. 1996,677, 305–312.CrossRefGoogle Scholar
  14. [14]
    Kreiser, W.R.; Martin, R.A. Jr.J. Assoc. Off. Anal. Chem. 1978,61, 1424–1427.Google Scholar
  15. [15]
    Tanaka, T.J. Chromatogr. A 1978,575, 311–315.Google Scholar
  16. [16]
    Raynal, J.; Moutounet, M.; Souquet, J.M.J. Agric. Food Chem. 1989,37, 1046–1050.CrossRefGoogle Scholar
  17. [17]
    Gasco-Lopez, A.I.; Santos-Montes, A.; Izquierdo-Hornillos, R.J. Chromatogr. Sci. 1997,35, 525–535.Google Scholar
  18. [18]
    Reta, M.; Carr P.W.J. Chromatogr. A 1999,855, 121–127.CrossRefGoogle Scholar
  19. [19]
    Liu, F.; Ascah, T.The Reporter, International Bulletin Supelco 1994,13, 2–5.Google Scholar
  20. [20]
    Ascah, T.; Liu F.The Reporter, International Bulletin Supelco 1995,14, 4–7.Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2000

Authors and Affiliations

  • M. Arlorio
    • 1
  • J. D. Coïsson
    • 1
  • A. Martelli
    • 1
  1. 1.Dipartimento di Scienze Chimiche, Alimentari, Farmaceutiche, Farmacologiche (DiSCAFF)Università del Piemonte Orientale “A. Avogadro”NovaraItaly

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