Advertisement

Chromatographia

, Volume 52, Issue 9–10, pp 552–558 | Cite as

Development of solid extraction unit for determining multiple herbicides in maize

  • A. Laganà
  • A. Bacaloni
  • G. Fago
  • A. Marino
Originals Column Liquid Chromatography

Summary

A multiclass method for the determination of five selected sulfonylurea, diphenylether, and sulfonanilide herbicides commonly used in maize growing has been developed.

In this study, the method development for the analysis was performed using a solids extraction unit for analyte extraction from maize. A procedure using solid phase extraction system for clean up the methanolic extract, and a liquid chromatography-tandem mass spectrometry (MS/MS) was developed for the final determination.

The extraction parameters that were studied included temperature, duration of the static extraction, sample amount, solvent volume, and flow rate. Maximum yield was obtained by extraction with 25 mL of pure methanol at 50 °C and a flow rate of 1 mL min−1. After extraction, herbicides present in methanolic phase were isolated and cleaned up by solid phase extraction using a reversed-phase C-18 cartridge and Carbograph-1 cartridge. The herbicides were chromatographically separated using reversed-phase HPLC and selectively analyzed by MS/MS using a positive ionization-turbo ion spray (PI-TISP) interface in multiple reaction monitoring (MRM) mode.

Key Words

Column liquid chromatography-tandem mass spectrometry Solid phase extraction Herbicides Maize 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    Hawthorne, S.B.; Yang, Y.; Miller, D.J.Anal. Chem. 1994,66, 2912CrossRefGoogle Scholar
  2. [2]
    CDS Tomlin Ed., The Pesticide Manual, 11th edition, British Crop Protection Council,1997.Google Scholar
  3. [3]
    Poole, C.F.; Poole, S.K.Anal. Comm. 1996,33, 11HGoogle Scholar
  4. [4]
    Yang, Y.; Hawthorne, S.B.; Miller, D.J.J. Environ. Sci. Technol. 1997,31, 430CrossRefGoogle Scholar
  5. [5]
    Crescenzi, C.; D’Ascenzo, G.; Di Corcia, A.; Nazzari, M.; Marchese, S.; Samperi, R.Anal. Chem. 1999,71, 2157CrossRefGoogle Scholar
  6. [6]
    Pawlowski, T.M.; Poole, C.F.J. Agric. Food Chem. 1998,46, 3124CrossRefGoogle Scholar
  7. [7]
    Nemoto, S.; Lehotay, S. J.J. Agric. Food Chem. 1998,46, 2190CrossRefGoogle Scholar
  8. [8]
    Yang, Y.; Li, B.Anal. Chem. 1999,71, 1491CrossRefGoogle Scholar
  9. [9]
    Crescenzi, C.; Di Corcia, A.; Passariello, G.; Samperi, R.; Turnes Carou, M.I.J. Chromatogr. 1996,733, 41CrossRefGoogle Scholar
  10. [10]
    Laganà, A.; Fago, G.; Marino, A.Anal. Chem. 1998,70, 121–130.CrossRefGoogle Scholar
  11. [11]
    Laganà, A.; Fago, G.; Marino, A.J. Chromatogr. A 1998,796, 309–318.CrossRefGoogle Scholar
  12. [12]
    Bucheli, T.D.; Grübler, F.C.; Müller, S.R.; Schwarzenbach, R.P.Anal. Chem. 1997,69, 1569CrossRefGoogle Scholar
  13. [13]
    Di Corcia, A.; Crescenzi, C.; Samperi, R.; Scappaticcio, L.Anal. Chem. 1997,69, 2819CrossRefGoogle Scholar
  14. [14]
    Frehse, H. InPesticide Science and Biotechnology (Proceedings of the 6 th IUPAC Congress of Pesticide Chemistry, Ottawa1986), Greenhalgh R.; Roberts, T. R., Eds., Blackwell, London.Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2000

Authors and Affiliations

  • A. Laganà
    • 1
  • A. Bacaloni
    • 1
  • G. Fago
    • 1
  • A. Marino
    • 1
  1. 1.Department of Chemistry«La Sapienza» UniversityRomaItaly

Personalised recommendations