Abstract
To investigate the manganese status in magnesium deficiency, 40 male Wistar rats, 3 wk old, were divided into two groups and fed a magnesium deficient diet or a normal synthetic diet for 2 wk. Dietary magnesium depletion decreased magnesium levels in brain, spinal cord, lung, spleen, kidney, testis, bone, blood, and plasma, while it elevated the magnesium level in liver. In magnesium-depleted rats, calcium concentration was increased in lung, liver, spleen, kidney, and testis, while it was decreased in tibia. In magnesium-depleted rats, manganese concentration was decreased in plasma and all tissues except adrenal glands and blood. Dietary magnesium depletion diminished pyruvate carboxylase (EC 6.4.1.1) activity in the crude mitochondrial fraction of liver. Positive correlation was found between the liver manganese concentration and the pyruvate carboxylase activity. In the magnesium-depleted rats, glucose was decreased while plasma lipids (triglycerides, phospholipids, and total cholesterol) were increased. These results suggest that dietary magnesium deficiency changes manganese metabolism in rats.
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References
M. S. Seelig and H. A. Heggtveit, Magnesium interrelationships in ischemic heart disease,Am. J. Clin. Nutr. 27, 59–79 (1974).
M. Kimura, T. Harada, and Y. Itokawa, Changes in blood pressure, body temperature, serotonin metabolism and tissue mineral levels in magnesium deficient rats,Magnesium (Kyoto) 2, 7–14 (1983) (in Japanese).
M. Kimura and Y. Itokawa Inefficient utilization of iron and minerals in magnesium deicient rats,Magnesium in Health and Diease, Y. Itokawa and J. Durlach, eds., John Libbey & Co Ltd, London & Paris, 1989, pp. 95–102.
B. M. Altura and B. T. Altura, Mg, Na and K interactions and coronary heart diseases,Magnesium 1, 241–265 (1982).
M. Kimura and K. Yokoi, Iron accumulation in tissues of magnesium deficient rats with dietary iron overload. Experimental hemochromatosis modelBiol. Trace Elem. Res. (in press).
L. S. Hurley and C. L. Keen, Manganese,Trace elements in human and animal nutrition, W. Mertz, eds., Academic, New York, pp. 185–223 (1987).
M. C. Scrutton, M. F. Utter, and A. S. Mildvan, Pyruvate caboxylase. VI The tightly bound manganese,J. Biol. Chem. 241, 3480–3487, (1966).
M. S. Mameesh and B. C. Johnson, The effect of penicillin on the intestinal synthesis of thiamine in the rat,J. Nutr. 65, 161–167 (1958).
M. C. Scrutton, M. R. Olmsted, and M. F. Utter, Pyruvate carboxylase from chicken liver,Methods Enzymol. 13, 235–249 (1969).
M. Kimura, M. Ujihara, and Y. Itokawa, Changes of manganese concentration in various tissues and pyruvate carboxylase activity in liver of magnesium deficient rats,Trace Nutrients Research (Kyoto) 7, 131–138 (1990) (Japanese).
H. D. Kruse, E. R. Orent, and E. V. McCollum, Studies on magnesium deficiency in animals. I. symptomatology resulting from magnesium deprivation,J. Biol. Chem. 96, 519–539 (1932).
Y. Itokawa and M. Fujiwara, Changes in tissue magnesium, calcium and phosphorus levels in magnesium deficient rats in relation to thiamin excess or deficiency,J. Nutr. 103, 438–443 (1973).
L. A. Goldsmith, Relative magnesium deficiency in the rat,J. Nutr. 93, 87–102 (1967).
N. W. Alcock and I. MacIntyre, Interrelation of calcium and magnesium,Clin. Sci. 22, 185–193 (1962).
T. Günter, Biochemistry of magnesium and calcium at membranes,Magnesium in Health and Disease, Y. Itokawa and J. Duriach, eds., John Libbey & Co Ltd, London & Paris, pp. 3–10 (1989).
M. J. Roby, K. L. Vann, J. H. Freeland-Graves, and R. L. Shorey, Plasma and liver cholesterol in the manganese-deficient rat,Fed. Proc. 41 786 (1982) (Abstract).
A. Matsuda, M. Kataoka, M. Sato, M. Kimura, and Y. Itokawa, Profile of manganese deficiency in rats,Biomed. Res. Trace Elements 4, 7–15 (1993) (Japanese).
M. C. Scrutton, P. Griminger, and J. C. Wallace, Pyruvate carboxylase. Bound metal cntent of the vertebrate liver enzyme as a function of diet,J. Biol. Chem. 247, 3305–3313 (1972).
L. R. McDowell,Minerals in Animal and Human Nutrition, Academic, New York (1992).
K. A. Dahlstrom, M. E. Ament, M. G. Medhin, and S. Meurling, Serum trace elements in children receiving long-term parenteral nutrition,J. Pediatr. 109, 625–630 (1986).
R. E. Burch, H. K. Hahn, and J. F. Sullivan, New aspects of the role of zinc, manganese and copper in human nutrition,Clin. Chem. 21, 501–520 (1975).
A. Matsuda, M. Kataoka, S. Ohkuma, M. Sato, and Y. Itokawa, Quantifying manganese in lymphocytes to assess manganese nutritional status,Clin. Chem. 35, 1939–1941 (1989).
A. Matsuda, M. Kimura, K. Yokoi, H. Kabata, Y. Itokawa, M. Kataoka, and M. Sato, Changes in trace-element concentrations after intravenous injection of an essential trace-element preparation for parenteral use in rats,Jpn. J. Hyg. 44, 887–893 (1989). (Japanese).
A. Matsuda, M. Kimura, K. Yokoi, H. Kabata, Y. Itokawa, M. Kataoka, and M. Sato, Effects of total parenteral nutrition containing essential trace elements on their concentrations in rats,Jpn. J. Hyg. 43, 1140–1148 (1989) (Japanese).
A. Matsuda, S. Kumadani, M. Kataoka, M. Sato, M. Kimura, and Y. Itokawa, Effects of an essential trace element agent (TE-5) for total parenteral nutrition on the mineral nutrition in rats fed a trace element-deficient diet,Jpn. J. Hyg. 47, 695–703 (1992). (Japanese).
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Kimura, M., Ujihara, M. & Yokoi, K. Tissue manganese levels and liver pyruvate carboxylase activity in magnesium-deficient rats. Biol Trace Elem Res 52, 171–179 (1996). https://doi.org/10.1007/BF02789459
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DOI: https://doi.org/10.1007/BF02789459