Biological Trace Element Research

, Volume 4, Issue 1, pp 1–19 | Cite as

The effect of albumin, ceruloplasmin, and other serum constitutents on Fe(II) oxidation

  • Franklin J. Carver
  • David L. Farb
  • Earl Frieden


This study has analyzed the role of several serum constituents, that have been proposed to effect the following reactionin situ: {fx1-1} {fx1-2} These reactions were monitored by measuring the rate of Fe(II) oxidation in the presence of apo-transferrin (reaction A) and Fe(III)-transferrin formation (reaction B) at 465 nm. Reactions A and B were found to be kinetically equivalent. The results show that, singly or in combination, bicarbonate, orthophosphate, citrate, apo-transferrin, and/or albumin have less than one-tenth of the ability to enhance the oxidation of Fe(II) compared to the serum enzyme, ceruloplasmin. It was also found that the rate of Fe(II) oxidation by serum Fe-ligands was influenced by the efficiency of oxygen utilization. Whereas ceruloplasmin produces a 4∶1 ratio of Fe(II) oxidized to oxygen utilized, the non-enzymic components yield a 2∶1 or 3.09∶1 ratio. These data support the role of ceruloplasmin as an antioxidant that prevents the formation of the intermediate active oxygen species O 2 · and H2O 2 · through the Fe(II) auto-oxidation reaction.

A hitherto unrecognized factor in the control of nonenzymic oxidation of Fe(II) was serum albumin. This protein, at >25 μM, was found to sharply dampen the rate of Fe(II) oxidation in the presence of a physiological concentration of bicarbonate, citrate, and transferrin Albumin did not appear to affect the ceruloplasmin catalyzed oxidation of Fe(II) at pH 7.0. The addition of ceruloplasmin effected up to a 44 × increase in the rate of Fe(II) oxidation and Fe(III)-transferrin formation even in the presence of 0.60 mM albumin.

Index Entries

Albumin, effect on serum Fe(II) oxidation ceruloplasmin, effect on serum Fe(II) oxidation serum factors, effect on Fe(II) oxidation Fe(II) oxidation, effect of albumin, ceruloplasmin, and serum factors on oxidation of serum Fe(II), effects of albumin, ceruloplasmin, and other factors on 


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Copyright information

© The Humana Press Inc 1982

Authors and Affiliations

  • Franklin J. Carver
    • 1
  • David L. Farb
    • 1
  • Earl Frieden
    • 1
  1. 1.Department of ChemistryFlorida State UniversityTallahassee

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