Bacitracin production by a new strain ofBacillus subtilis

Extraction, purification, and characterization
  • E. C. Azevedo
  • E. M. Rios
  • K. Fukushima
  • G. M. Campos-Takaki


A new strain ofBacillus subtilis C 126 was isolated from sugar cane fermentation and produced an antibiotic that inhibited the growth ofMicrococcus flavus. The production of the antibiotic in culture medium followed to extraction withn-butanol, thin layer chromatography, and microbiological tests indicated that a polypeptide antibiotic was produced. The fraction obtained by Sephadex G-25 column and analyzed by HPLC indicated that bacitracin complex was produced.

Index Entries

Bacillus subtilis bacitracin production Micrococcus flavus 


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Copyright information

© Humana Press Inc. 1993

Authors and Affiliations

  • E. C. Azevedo
    • 1
  • E. M. Rios
    • 2
  • K. Fukushima
    • 3
  • G. M. Campos-Takaki
    • 2
    • 3
  1. 1.Mestrado de Ciencias FarmacÊuticasUniversidade Federal de PernambucoRecife-PEBrazil
  2. 2.Depto. de AntibióticosUniversidade Federal de PernambucoRecife-PEBrazil
  3. 3.Laboratório de Imunopatologia Prof. Keizo Asami-LIKAUniversidade Federal de PernambucoRecife-PEBrazil

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