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Acid Pullulanase from Bacillus polymyxa MIR-23

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Abstract

Within the frame of a screening program aimed at the isolation of amylolytic sporeformers, one strain with high amylolytic activity designated MIR-23 was selected. The microbial characterization was carried out by morphological and biochemical tests and, by means of statistical treatment, was identified asBacillus polymyxa. The organism could grow in acidic conditions (pH 5.0) on a starch medium and produce α-amylase, pullulanase, and α-glucosidase. Batch cultures showed the highest enzyme activities in the stationary phase. Pullulanase activity exhibited an optimal temperature of 52–57°C at pH 4.5–5.5. These properties would allow its use in the saccharification processes in the starch industries.

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Authors and Affiliations

  1. Cátedra de Microbiologia Superior, Facultad de Bioquimica, Quimica y Farmacia, Universidad Nacional de Tucuman, PROIMI-MIRCEN. Avda. Belgrano y Caseros, 4000, Tucumán

    Guillermo R. Castro, Luis M. Ducrey Santopietro & Faustino Siñeriz

  2. Laboratorio de Microbiologia, Departamento de Quimica Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 1428, Buenos Aires, Argentina

    Guillermo R. Castro & Luis M. Ducrey Santopietro

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  1. Guillermo R. Castro
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  2. Luis M. Ducrey Santopietro
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  3. Faustino Siñeriz
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Castro, G.R., Santopietro, L.M.D. & Siñeriz, F. Acid Pullulanase from Bacillus polymyxa MIR-23. Appl Biochem Biotechnol 37, 227–233 (1992). https://doi.org/10.1007/BF02788874

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  • DOI: https://doi.org/10.1007/BF02788874

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