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Extraction of L-Lysine from Its Dilute Aqueous Solutions by Rotating Film Pertraction

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Abstract

Removal and concentration of L-lysine in a three liquid phase pertraction system was studied using a laboratory three stage rotating disk pertractor. A 5% (vol) solution of Di-2-ethyl hexyl phosphoric acid (D2EHPA) in normal paraffins was used as an intermediate membrane liquid. The amino acid was extracted from its dilute aqueous solution (feed F) and concentrated in the stripping liquid R—a 1N solution of HCl in water. D2EHPA dissolved in the membrane liquid played the role of a ion exchange carrier. It was found that the most important parameters controlling the efficiency of this continuous pertraction process are the speed of disk rotation and the feed solution flowrate. The counter-current flow arrangement in the apparatus and the high flowrate ratio chosen provided nearly complete removal of solute from the feed as well as a concentrated solution of L-lysine.

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References

  1. Higgins, I. J., Best, D. J., and Jones, J. (1985),Biotechnology principles and applications, Blackwell Scientific, London.

    Google Scholar 

  2. Thien, M. and Hatton, T. (1988),Sep. Sci. & Technol. 23, 819.

    Article  CAS  Google Scholar 

  3. Bailly, J. E. and Olis, D. F. (1987),Biochemical engineering fundamentals, vol. 2, McGraw-Hill, New York, p. 406.

    Google Scholar 

  4. Thien, M., Hatton, T., and Wang, D. (1988),Biotech. Bioeng. 32, 604.

    Article  CAS  Google Scholar 

  5. Scheper, T., Likidis, Z., Makryaleas, K., Nowottny, C., and Schuegerl, K. (1987),Enzyme Microb. Tech. 9, 625.

    Article  CAS  Google Scholar 

  6. Yagodin, G., Yurtov, E., and Golubkov, A. (1986),Proceed. ISEC’86 3, 677.

    Google Scholar 

  7. Boyadzhiev, L. and Atanassova, I. (1991),Biotech. Bioeng. 38, 1059.

    Article  CAS  Google Scholar 

  8. Boyadzhiev, L. (1990),Sep. Sci. & Technol. 25, 187.

    Article  CAS  Google Scholar 

  9. Schlosser, S. and Kossaczky, E. (1986),J. Radional. Nucl. Chem. 101, 115.

    Article  CAS  Google Scholar 

  10. Boyadzhiev, L. and Alexandrova, S.Sep. Sci. & Technol. (in press).

  11. Boyadzhiev, L. and Bezenshek, E. (1988),J. Membr. Sci. 37, 277.

    Article  CAS  Google Scholar 

  12. Roberts, J. and Caserio, M. (1964),Basic Principles of organic chemistry, W.A. Benjamin, New York.

    Google Scholar 

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Boyadzhiev, L., Atanassova, I. Extraction of L-Lysine from Its Dilute Aqueous Solutions by Rotating Film Pertraction. Appl Biochem Biotechnol 37, 89–95 (1992). https://doi.org/10.1007/BF02788860

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  • DOI: https://doi.org/10.1007/BF02788860

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