Applied Biochemistry and Biotechnology

, Volume 37, Issue 1, pp 81–88 | Cite as

Protoplast Fusion of β-Glucosidase-Producing Aspergillus niger Strains

  • Yin Klong Hoh
  • Teck Koon Tan
  • Hock-Hin Yeoh


Protoplast fusion, induced by polyethylene glycol and Ca2+, was carried out between two auxotrophic strains ofAspergillus niger. The fusion frequency ranged from 6.2 × 10-2-9.1 × 10-2. After induced haploidization of a diploid, various segregants showing combinations of the parental genetic markers were isolated. Unlike diploids, haploid segregants exhibited greater variations in their morphology and β-glucosidase activities. One segregant showed a 2.5-fold increase in β-glucosidase activity over those of the parents. Thus, this method appears promising for creating new recombinant strains ofA. niger with improved β-glucosidase activities.

Index Entries

β-glucosidase cellobiose hydrolysis protoplast fusion Aspergillus niger 


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Copyright information

© The Humana Press Inc 1992

Authors and Affiliations

  • Yin Klong Hoh
    • 1
  • Teck Koon Tan
    • 1
  • Hock-Hin Yeoh
    • 1
  1. 1.Department of Botany, Faculty of ScienceNational University of SingaporeSingapore

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