Advertisement

Applied Biochemistry and Biotechnology

, Volume 37, Issue 1, pp 81–88 | Cite as

Protoplast Fusion of β-Glucosidase-Producing Aspergillus niger Strains

  • Yin Klong Hoh
  • Teck Koon Tan
  • Hock-Hin Yeoh
Article

Abstract

Protoplast fusion, induced by polyethylene glycol and Ca2+, was carried out between two auxotrophic strains ofAspergillus niger. The fusion frequency ranged from 6.2 × 10-2-9.1 × 10-2. After induced haploidization of a diploid, various segregants showing combinations of the parental genetic markers were isolated. Unlike diploids, haploid segregants exhibited greater variations in their morphology and β-glucosidase activities. One segregant showed a 2.5-fold increase in β-glucosidase activity over those of the parents. Thus, this method appears promising for creating new recombinant strains ofA. niger with improved β-glucosidase activities.

Index Entries

β-glucosidase cellobiose hydrolysis protoplast fusion Aspergillus niger 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Mandels, M. (1982),Ann. Rpt. Ferment. Process. 5, 35.Google Scholar
  2. 2.
    Friedrich, J., Cimerman, A., and Perdih, A. (1987),Appl. Microbiol. Biotechnol. 26, 299.CrossRefGoogle Scholar
  3. 3.
    Helmi, S., Khalil, A. I., Tahoun, M. K., and Khairy, A. H. (1991),Appl. Biochetn. Biotechnol. 28/29, 203.Google Scholar
  4. 4.
    Kirimura K., Lee, S. P., Nakajima, I., Kawabe, S., and Usami, S. (1988),J. Ferment. Technol. 66, 375.CrossRefGoogle Scholar
  5. 5.
    Pontecorvo, G., Roper, J. A., Hemmons, L. M., McDonald, K. D., and Bufton, A. W. J. (1953),Adv. Genet. 5, 142.Google Scholar
  6. 6.
    Hoh, Y. K., Yeoh, H. H., and Tan, T. K. (1992),Appl. Microbiol. Biotechnol. (in press).Google Scholar
  7. 7.
    Hamlyn, P. F., Bradshaw, R. E., Mellon, F. M., Santiago, C. M., Wilson, J. M., and Peberdy, J. F. (1981),Enzyme Microb. Technol. 3, 321.CrossRefGoogle Scholar
  8. 8.
    Kirimura, K., Yaguchi, T., and Usami, S. (1986),J. Ferment. Technol. 64, 473.CrossRefGoogle Scholar
  9. 9.
    Anne, J. and Peberdy, J. F. (1976),J. Gen. Microbiol. 92, 413.Google Scholar
  10. 10.
    Roper, J. A. (1952),Experientia 8, 14.CrossRefGoogle Scholar
  11. 11.
    Hastie, A. C. (1970),Nature 226, 771.CrossRefGoogle Scholar
  12. 12.
    Herbert, D., Phipps, P. J., and Strange, R. E. (1971), inMethods of Microbiology, vol. 5B, Norris, J. R. and Ribbons, D. W., eds., Academic, New York, pp. 322–328.Google Scholar
  13. 13.
    Burton, K. (1956),Biochem. J. 62, 315.Google Scholar
  14. 14.
    Latt, S. A. (1973),Proc. Natl. Acad. Sci. USA 70, 3395.CrossRefGoogle Scholar
  15. 15.
    Hedrick, J. L. and Smith, A. J. (1968),Arch. Biochem. Biophys. 126, 155.CrossRefGoogle Scholar
  16. 16.
    Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951),J. Biol. Chem. 193, 265.Google Scholar
  17. 17.
    Bailey, J. L. (1967), inTechniques in Protein Chemistry, Elsevier, Amsterdam, p. 346.Google Scholar
  18. 18.
    Cao, J. W. and Chen, S. Y. (1988),FEMS Microbiol. Lett. 50, 1.CrossRefGoogle Scholar
  19. 19.
    Peberdy, J. F. (1985), inFungal Protoplasts. Applications in Biochemistry and Genetics Peberdy, J. F. and Ferenczy, L., eds., Marcel Dekker, New York, Basel, pp. 31–44.Google Scholar
  20. 20.
    Stagg, C. M. and Feather, M. S. (1973),Biochim. Biophys. Acta 320, 64.Google Scholar
  21. 21.
    Peberdy, J. F. (1989),Mycol. Res. 93, 1.CrossRefGoogle Scholar
  22. 22.
    Friedrich, J., Cimerman, A., and Perdih, A. (1983),Eur. J. Appl. Microbiol. Biotechnol. 17, 243.CrossRefGoogle Scholar
  23. 23.
    Gunde-Cimerman, N., Cimerman, A., and Perdih, A. (1986),Enzyme Microb. Technol. 8, 166.CrossRefGoogle Scholar
  24. 24.
    Gokhale, D. V., Puntambekar, U. S., Deobagkar, D. N., and Peberdy, J. F. (1988),Enzyme Microb. Technol. 10, 442.CrossRefGoogle Scholar

Copyright information

© The Humana Press Inc 1992

Authors and Affiliations

  • Yin Klong Hoh
    • 1
  • Teck Koon Tan
    • 1
  • Hock-Hin Yeoh
    • 1
  1. 1.Department of Botany, Faculty of ScienceNational University of SingaporeSingapore

Personalised recommendations