Advertisement

Applied Biochemistry and Biotechnology

, Volume 37, Issue 1, pp 11–17 | Cite as

Protection of aspergillus niger cellulases by urea during growth on glucose or glycerol supplemented media

  • D. V. Gokhale
  • S. G. Patil
  • K. B. Bastawde
Article

Abstract

The cellulase enzymes ofAspergillus niger were found to undergo catabolite repression in the presence of glucose and glycerol accompanied by sudden drop in pH of the fermentation medium below 2.0. This sudden drop in pH caused inactivation of cellulolytic enzymes produced byAspergillus niger. The supplementation of nitrogen sources, especially urea, protectsA. niger cellulases from inactivation caused by a sudden drop in pH, since urea helped to maintain the pH of the fermentation medium between 3.5 and 4.5. The role of urea in the protection of cellulase was more prominent when it was used in combination with glycerol (5%).

Index Entries

Catabolite repression Aspergillus niger urea cellulases glucose and glycerol pH inactivation 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Egilsson, V., Gudnason, V., Jonasdottir, A., Ingvarsson, S., and Andresdottir, V. (1986),J. Gen. Microbiol. 132, 3309–3313.Google Scholar
  2. 2.
    Hsu, E. and Vaughn, R. (1969),J. Bacteriol. 98, 172–181.Google Scholar
  3. 3.
    Kilian, S., Prior, B., and Latigan, P. (1983),Eur. J. Appl. Microbiol. Biotechnol. 18, 369–373.CrossRefGoogle Scholar
  4. 4.
    Maldonado, M. C., Navarro, A. R., and Callieri, D. A., (1986),Biotechnol. Lett. 8, 501–504.CrossRefGoogle Scholar
  5. 5.
    Coughlan, M. P.. (1985),Biotechnol. Gen. Eng. Rev. 3, 39–109.Google Scholar
  6. 6.
    Gong, G. S. and Tsao, G. T.. (1979),Ann. Rep. Ferment. Proc. 2, 111–140.Google Scholar
  7. 7.
    Judewicz, N. and Kornblihtt, A. R.. (1983),Bioquimica General De Torres, Ateneo, E. I., ed., Carminatti, Cardini, pp. 843–844.Google Scholar
  8. 8.
    Shinmyo, A., Mitsushima, K., and Terui, G. (1972),J. Ferment. Technol. 50, 647–654.Google Scholar
  9. 9.
    Shinmyo, A., Davis, I. K., Nomoto, F., Tahara, T., and Enatsu, T. (1978),Eur. J. Appl. Microbiol. Biotechnol. 5, 59–68.CrossRefGoogle Scholar
  10. 10.
    Tahara, T., Doi, S., Shinmyo, A., and Terui, G. (1972),J. Ferment. Technol. 50, 655–661.Google Scholar
  11. 11.
    Reese, E. T. and Mandels, M. (1971),High Polymers 5, 1079–1094.Google Scholar
  12. 11.
    Bagga, P. S., Sandhu, D. K., and Sharma, S. (1989),Proc. Biochem. 24, 41–45.Google Scholar
  13. 13.
    Herr, D.(1979),Biotechnol. Bioeng. 21, 1361–1371.CrossRefGoogle Scholar
  14. 14.
    Horton, J. C. and Keen, N. T.. (1966),Can. J. Microbiol. 12, 209–220.CrossRefGoogle Scholar
  15. 15.
    Karla, M. K., Sidhu, M. S., and Sandhu, D. K.. (1984),Appl. Microbiol. Biotechnol. 20, 427–429.Google Scholar
  16. 16.
    Karla, M. K., Sidhu, M. S., and Sandhu, D. K.. (1986),J. Appl. Bacteriol. 61, 71–80.Google Scholar
  17. 17.
    Sandhu, D. K. and Sidhu, M. S.. (1985),J. Basic Microbiol. 25, 591–598.CrossRefGoogle Scholar
  18. 18.
    Sidhu, M. S. and Sandhu, D. K.. (1984),Can. J. Microbiol. 30, 1377–1382.CrossRefGoogle Scholar
  19. 19.
    Gokhale, D. V., Puntambekar, U. S., Vyas, A. K., Patil, S. G., and Deobagkar, D. N.. (1984),Biotechnol. Lett. 6, 719–722.CrossRefGoogle Scholar
  20. 20.
    Gokhale, D. V., Puntambekar, U. S., and Deobagkar, D. N., (1986),Biotechnol. Lett. 8, 37–38.CrossRefGoogle Scholar
  21. 21.
    Gokhale, D. V., Puntambekar, U. S., Deobagkar, D. N., and Peberdy, J. F., (1988),Enzyme Microb. Technol. 10, 442–445.CrossRefGoogle Scholar
  22. 22.
    Gokhale, D. V., Patil, S. G., and Bastawde, K. B.. (1991),Appl. Biochem. Biotechnol. 30, 99–109.CrossRefGoogle Scholar
  23. 23.
    Reese, E. T. and Mandels, M. (1963),Meth. Carbohydr. Chem. 3, 139–143.Google Scholar
  24. 14.
    Fischer, E. H. and Stein, E. A.. (1961),Biochem. Prep. 8, 32–34.Google Scholar
  25. 15.
    Borgia, P. and Shepherd, P. S.. (1977),J. Bacteriol. 130, 812–817.Google Scholar
  26. 16.
    Manning, K. and Wood, D. A.. (1983),J. Gen. Microbiol. 129, 1835–1847.Google Scholar
  27. 27.
    Umezurike, G. M.. (1981),Biochem. J. 199, 203–208.Google Scholar
  28. 28.
    Grajeck, W. (1987),Enzyme Microb. Technol. 9, 744–748.CrossRefGoogle Scholar

Copyright information

© The Humana Press Inc 1992

Authors and Affiliations

  • D. V. Gokhale
    • 1
  • S. G. Patil
    • 1
  • K. B. Bastawde
    • 1
  1. 1.NCIM Division of Biochemical SciencesNational Chemical LaboratoryPuneIndia

Personalised recommendations