Abstract
The influence of potassium on ethanol production bySaccharomyces cerevisiae wild type and AR5 cells carrying the plasmid pCYG4 was investigated. This plasmid carries the glutamate dehydrogenase gene conferring an 11-fold higher level of expressed enzyme activity over the wild type cells. All experiments were carried out in batch culture with medium supplemented to different potassium concentrations up to 180 mM. Maximum ethanol production rate was observed in the AR5 cells grown in medium supplemented with 3.5 mM of potassium ions. Glucose uptake rate increased with increasing potassium up to 60 mM, but higher concentrations depressed glucose uptake rate in both strains. Furthermore, the wild type cells showed higher growth rate, ethanol production, and glucose consumption rate than the AR5 cells. These lower rates in the AR5 cells could be explained by repression of potassium uptake by an enhancement of ammonium feeding, and greater energy requirements by these cells due the presence of the plasmid.
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Kappeli, O. Institute of Biotechnology, Swiss Federal Institute of Technology, Hoenggerberg, 8093, Zurich, Switzerland, pp.181–208.
Pascual, C, Alonso, A., Garcia, I., Romay, C, and Kotyk, A. (1988),Biotech. Bioeng. 32, 374–378.
De La Pena, P., Barros, F., Gascon, S., Ramos, S., Lazo, P. S. (1982),Eur. J. Biochem. 123, 447–453.
Jimenez, J. and Van Uden, N. (1985),Biotech. Bioeng. 27, 1596–1598.
Camacho, M., Ramos, J., and Navarro, A. R.. (1981),Current Microbiology 6, 295–299.
Ramos, J., Contreras, P., and Navarro, A. R.. (1985),Arch. Microbiol. 143, 88–93.
Wimpelmann, M., Kjaergarrd, L., and Joergensen, B. B.. (1984),Biotech. Bioeng. 26, 301–307.
Pena, A., Mora, M. A., and Carrasco, N. (1979),J. Memb. Biol. 47, 261–284.
Bogonez, J. M., Greson, M., and Arst, H. N.. (1986),Adv. Microb. Physiol. 26, 1–87.
Gonzalez, A., Rodrigues, L., Oliveira, H., and Soberon, M. (1985),J. Gen. Microb. 131, 2565–2571.
Wiame, J. M., Greson, M., and Arst, H. N.. (1986),Adv. Microb. Physiol. 26, 1–87.
Lima-Filho, J. L. and Ledingham, W. M.. (1990),Appl. Biochem. Biotech. 23, 181–186.
Lima-Filho, J. L. and Ledingham, W. M.. (1988),Appl. Biochem. Biotech. 19, 27–32.
Bernfield, P. (1955),Methods of Enzymology 1 Collowick, S. P. and Kaplan, N. O., eds., Academic, New York, pp. 149–150.
Lima Filho, J. L.. (1987), Ph.D. diss., University of St. Andrews, Scotland, UK.
Navarro, A. R. and Ramos, J. (1984),J. Gen. Bacteriol.,3 940–945.
Thomas, D. S. and Rose, A. H. (1979),Arch. Microbiol. 122, 49–55.
Leao, C. and VanUden, N. (1983),Biotech. Bioeng. 25, 2085–2090.
Pena, A., Cinco, Garcia, A., Gomez Puyou, A., and Tuena, M. M.. (1967),Biochem. Biophys. Acta 148, 673–682.
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Da Silva, N.L.C.L., Salgueiro, A.A., Ledingham, W.M. et al. Effects of potassium on the ethanol production rate of Saccharomyces cerevisiae carrying the plasmid pCYG4 related with ammonia assimilation. Appl Biochem Biotechnol 37, 1–10 (1992). https://doi.org/10.1007/BF02788852
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DOI: https://doi.org/10.1007/BF02788852