Abstract
The pretreatment of corn fiber using liquid water at temperatures between 220 and 260°C enhances enzymatic hydrolysis. This paper describes the laboratory reactor system currently in use for cooking of corn fiber at temperatures ranging from 200 to 260°C. The corn fiber at approx 4.4% solid/liquid slurry was treated in a 2-L, 304 SS, Parr reactor with three turbine propeller agitators and a Proportional-Integral-Derivative (PID), controller that controlled temperature within ±1°C. Heat-up times to the final temperatures of 220, 240, or 260°C were achieved in 50 to 60 min. Hold time at the final temperature was less than 10 s. A serpentine cooling coil, through which tap water was circulated at the completion of the run, cooled the reactor’s contents to 180°C within 2 min after the maximum temperature was attained. Ports in the reactor’s head plate facilitated sampling of the slurry and monitoring the pH. A continuous pH monitoring system was developed to help observe trends in pH during pretreatment and to assist in the development of a base (2.0M KOH) addition profile to help keep the pH within the range of 5.0 to 7.0. Enzymatic hydrolysis gave 33 to 84% conversion of cellulose in the pretreated fiber to glucose compared to 17% for untreated fiber.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bobleter, O., Niesner, R., and Rohr, M. (1976),J. Appl. Polymer Sci. 20, 2083–2093.
Haw, J. F., Maciel, G. E., Linden, J. C., and Murphy, V. G. (1985),Holzforschung 39, 99–107.
Hormeyer, H. F., Schwald, W., Bonn, G., and Bobleter, O. (1988),Holzforschung 42(2), 95–98.
Walch, E., Zemann, A., Schinner, F., Bonn, G., and Bobleter, O. (1992),Biores. Technol. 39, 173–177.
Mok, W. S-L. and Antal, M. J. Jr. (1992),Ind. Eng. Chem. Res. 31, 1157–1161.
vanWalsum, G. P., Allen, S. G., Spencer, M. J., Laser, M. S., Antal, M. J. Jr., and Lynd, L. R. (1996),Appl. Biochem. Biotechnol. 57/58, 157–170.
Foody, P. (July 24,1984), US patent # 4,461,648.
Beltrame, P. L., Carniti, P., Visciglio, A., Focher, B., and Marzett, A. (1992),Biores. Technol. 39, 165–171.
Heitz, M., Capek-Menard, E., Koeberle, P. G., Gagne, J., Chornet, E., Overend, R. P., Taylor, J. D., and Yu, E. (1991),Biores. Technol. 35, 23–32.
Brownell, H. H. and Saddler, J. N. (1987),Biotechnol. Bioeng. 29, 228–235.
Ladisch, M. R., Waugh, L., Westgate, P., Kohlmann, K., Hendrickson, R., Yang, Y., and Ladisch, C. (1992), in Ladisch, M. R. and Bose, A., eds., American Chemical Society, Washington, DC, 510-518.
Kohlmann, K. L., Westgate, P. J., Weil, J., and Ladisch, M. R. (1994),J. Aerospace 102, 1476–1483.
Weil, J., Westgate, P., Kohlmann, K., and Ladisch, M. R. (1994),Enzyme Microb. Technol. 16, 1002–1004.
Kohlmann, K. L., Sarikaya, A., Westgate, P. J., Weil, J., Velayudhan, A., Hendrickson, R., and Ladisch, M. R. (1995), in Penner, M. and Saddler, J., eds., American Chemical Society, Washington, DC, No. 618, 237-255.
Kohlmann, K. L., Westgate, P., Velahudhan, A., Weil, J., Sarikaya, A., Brewer, M. A., Hendrickson, R. L., and Ladisch, M. R. (1996),Adv. Space Res. 18(1/2), 251–265.
NREL, Chemical Analysis and Testing Standard Procedures, Golden, CO (1994).
Baugh, K. D. and McCarty, P. L. (1988),Biotechnol. Bioeng. 31, 50–61.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Weil, J.R., Sarikaya, A., Rau, SL. et al. Pretreatment of corn fiber by pressure cooking in water. Appl Biochem Biotechnol 73, 1–17 (1998). https://doi.org/10.1007/BF02788829
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02788829