Applied Biochemistry and Biotechnology

, Volume 66, Issue 1, pp 31–37 | Cite as

The effect of formaldehyde on the growth ofCandida diddensii 74-10 andCandida tropicalis R-70

  • Christina Katranushkova
  • Bonka Tzvetkova
  • Lila Losseva


The effects of formaldehyde on the growth of two strains of fodder yeastsCandida diddensii 74-10 andCandida tropicalis R-70 were investigated using the method of continuous cultivation under conditions of carbon limitation and at dilution rates of 0.1/h and 0.25/h. The results indicate that formaldehyde induces a decrease in the yield of biomass, but stimulates the synthesis of protein and RNA. The authors studied the activities of the following enzymes: NADPH-linked glutamate dehydrogenase, NADH-linked glutamate dehydrogenase, alanine dehydrogenase, glutamine synthetase, and glutamate synthase, which are utilized in nitrogen metabolism. The data obtained showed an increase in the activity of the glutamate dehydrogenase pathway of ammonium assimilation. It was also established that formaldehyde caused considerable changes in the micro-organisms at the higher dilution rate.

Index entries

Formaldehyde Candida tropicalis Candida diddensii nitrogen metabolism protein continuous cultivation 


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Copyright information

© Humana Press Inc. 1997

Authors and Affiliations

  • Christina Katranushkova
    • 1
  • Bonka Tzvetkova
    • 1
  • Lila Losseva
    • 1
  1. 1.Institute of MicrobiologyBulgarian Academy of SciencesSofiaBulgaria

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