Abstract
In the acute phase of Chagas’ disease, when the parasitemia is high, diagnosis can be easily made using conventional parasitological methods. During the chronic phase, due to the low parasitemia, diagnosis is performed mainly by immunological methods. Conventional serological techniques are limited by cross-reactivity with other parasitic diseases, non-standardization of reagents, and the diversity of technical procedures. Methods are being developed to improve the sensitivity and specificity of diagnosis using molecular approaches. PCR-based detection systems and the use of recombinant antigens in ELISA are the most promising.
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References
WHO (1991),Control of Chagas’ Disease. Report of a WHO Expert Committee, 97.
Brener, Z. (1987),Mem. Inst. Oswaldo Cruz 82, 205–213.
Carrasco, R. L., Miguez, H. V., Camacho, C., Ehalar, L., Rivollo, S., Ampuero, T. and Dedet, J. P. (1990),Mem. Inst. Oswaldo Cruz 85, 69–73.
Schimunis, G. A. (1991),Transfusion 31, 547–557.
Morgado, M. G., Ivodos Santos J., Arguelles, B., and GalvÃo-Castro, B. (1989),Mem. Inst. Oswaldo Cruz. 84, 309–314.
Andrade, S. G. (1985),Rev. Soc. Bras. Med. Trop. 18, 39–16.
Gomes, Y. M., Leal, T. C. A., Silva, M. R., Santiago, C. M. G., and Coutinho, E. M., (1995),Rev. Inst. Med. Trop. 37, 87–89.
Morgado, M. G., van Hoegaerden M., and GalvÃo-Castro, B. (1985),Z. Parasitenkd. 71, 169–178.
Camargo, E. M. and Takeda, G. K. F. (1979),Trypanosoma cruzi e DoenÇa de Chagas, Brener, Z. and Andrade, Z. eds., Guanabara Koogan, Rio de Janeiro, Brazil.
Camargo, M. E. and Rebonato, C. (1969),Am. J. Trop. Med. Hyg. 18, 500–505.
Anthony, R. L., Johnson, C. M., and Souza, O. E. (1979),Am. J. Trop. Med. Hyg. 28, 969–973.
Carrasco, R. L., Breniere, S. F., Poch, O., Miguez, H. V., Selaes, H., Anatezana, G., Desjeux, P., and Carlier, Y. (1985),Ann. Soc. Belg. Med. Trop. 65, 79–84.
Carvalho, M. R., Krieger, M. A., Almeida, E., Oelemann, W., Duartedos Santos, C.N., and Goldenberg, S. (1992),Mem. Inst. Oswaldo Cruz. 2, 134.
Velazquez, E., Reyes, L., Thors, C., Miettien, A., Chinchilla, M., and Linder, E. (1993),Trans. R. Soc. Trop. Med. Hyg. 87, 35.
Minoprio, P., Itohara, S., Heusser, C., Tonegawa, S., and Coutinho, A. (1989),Immunol. Rev. 112, 183–187.
Orozco, O., Afchain, D., Dissous, C., Rodrigues, C., Ovlaque, G., Lemesre, J. L., Loyens, M., and Capron, A. (1984),Am. J. Trop. Med. Hyg. 33, 560–568.
Afchain, D., Le Ray, Fruit, D., and Capron, A. (1979),J. Parasitol. 65, 507–514.
Lemesre, J. L., Afchain, D., Orozco, O., Loyens, M., Breniere, F. S., Desjeux, P., Carlier, Y., Martin, U., NogueiraQueiroz A., LeRay D., and Capron, A. (1989),Am. J. Trop. Med. Hyg. 35, 86–93.
Tachibana, H., Montenegro, L. T., Kurihara, K., Nagakura, K., Kaneda, Y., and Komasu, N. (1986),Z. Parasitenkd. 72, 701–707.
Tachibana, H., Nagakura, K., and Kaneda, Y. (1988),Parasitol. Res. 74, 409–414.
Ouaissi, M. A., Taibi, J. A., Cornette, J., Velge, P., Marty, B., Loyens, M., Esteva, M., Rizvi, F. S., and Capron, A. (1990),Parasitology 100, 115–124.
Bergquist, N. R. (1990),Mem. Inst. Oswaldo Cruz 87, 29–38.
Kirchhoff, L. V., Gam, A. A., d’AGusmÃo R., Goldsmith, R. S., Rezende, J. M., and Rassi, A. (1987),J. Infect. Dis. 155, 561–564.
Cooper, R., Inverso, J. A., Espinosa, M., Nogueira, N., and Cross, G. A. M. (1991),Mol. Biochem. Parasitol. 49, 45–60.
Zingales, B., Gruber, A., Ramalho, C. B., Umezawa, E. S., and Coli, W. (1990),Mem. Inst. Oswaldo Cruz 85, 519–522.
Ibanez, C. F., Affranchino, J. L., Macina, R. A., Reyes, M. B., Leguizamon, S., Camargo, M. E., Aslund, L., Petterson, U., and Frash, A. C. C. (1988),Mol. Biochem. Parasitol. 30, 27–34.
Hoft, D. F., Kim, K. S., Otsu, K., Moser, D. R., Yost, W. J., Blumin, J. H., Donelsos, J. E., and Kirchhoff, L. V. (1989),Infect. Immunol. 57, 1959–1967.
Levin, M. J., Mesri, E., Benarous, R., Levitus, G., Schijman, A., Levy-Yeati, P., Chiale, P.A., Ruiz, A. M., Kahn, A., and Rosenbaum, M. B. (1990),Am. J. Trop. Med. Hyg. 41, 530–538.
Goldenberg, S., Krieger, M. A., Lafaille, J. J., Almeida, E., and Oelemann, W. (1991),Mem. Inst. Butantan. 53, 71–76.
Frash, A. C. C., Cazzullo, J. J., Aslund, L., and Petterson, U. (1991),Parasitol. Today 7, 148–151.
Burns, J. M., Jr., Shreffler, W. G., Rosman, D. E., Sleath, P. R., March, C. J., and Reed, S.G. (1992),Proc. Natl. Acad. Sci. 89, 1239–1243.
Moncayo, A. and Luquetti, A. O. (1990),Mem. Inst. Oswaldo Cruz 85, 489–495.
Lafaille, J. J., Linss, J., Krieger, M. A., Souto-Padron, T., Desouza W., and Goldenberg, S. (1989),Mol. Biochem. Parasitol. 35, 127–137.
Krieger, M. A., Almeida, E., Oelemann, W., Lafaille, J. J., Borges-Pereira, J., Kreger, H., Carvalho, M. R., and Goldenberg, S. (1992),Am. J. Trop. Med. Hyg. 46, 427–434.
Simpson, L. (1987),Annu. Rev. Microb. 41, 363–382.
Degrave, W., Fragoso, S., Britto, C., Van Heuverswyn H., Kidane, G. Z., Cardoso, M. A.B., Mueller, R. V., Simpson, L., and Morel, C. M. (1988),Mol. Biochem. Parasitol. 27, 63–70.
Degrave, W., Fernandes, O., Thiemann, O., Winker, P., Britto, C., Cardoso, A., BorgesPereira, J., Bozza, M., Lopes, V., and Morel, C. M. (1994),Mem. Inst. Oswaldo Cruz 89, 367, 368.
Sturn, N. R., Degrave, W., Morel, C. M., and Simpson, L. (1989),Mol. Biochem. Parasitol. 33, 205–214.
Avila, H. A., Sigman, D. S., Cohen, L. M., Millikan, R. C., and Simpson, L. (1991),Mol. Biochem. Parasitol. 48, 211–222.
Moser, D. R., Kirchhoff, L. V., and Donelson, J. E. (1989),J. Clin. Microbiol. 27, 1477–1482.
Dias, C., Nussenzweig, V., and Gonzalez, A. (1992),Am. J. Trop. Med. Hyg. 46, 616–623.
Britto, C., Cardoso, M. A., Wincker, P., and Morel, C. M. (1993),Mem. Inst. Oswaldo Cruz 88, 171, 172.
Wincker, P., Britto, C., BorgesPereira J., Cardoso, A., Oelemann, W., and Morel, C. M. (1994),Am. J. Trop. Med. Hyg. 51, 771–777.
Britto, C., Cardoso, M. A., Vanny, C. M. M., HasslocherMoreno, A., Xavier, S. S., Oelemann, W., Santoro, A., Piermez, C., Morel, C. M., and Wincker, P. (1995),Parasitology 110, 241–247.
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Gomes, Y.M. PCR and sero-diagnosis of chronic chagas’ disease biotechnological advances. Appl Biochem Biotechnol 66, 107–119 (1997). https://doi.org/10.1007/BF02788756
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DOI: https://doi.org/10.1007/BF02788756