Applied Biochemistry and Biotechnology

, Volume 47, Issue 1, pp 65–72 | Cite as

L-malic acid production by entrappedSaccharomyces cerevisiae into polyacrylamide gel beads

  • Elzabeth A. Oliveira
  • Aurelio A. R. Costa
  • Zilda M. B. Figueiredo
  • Luiz B. Carvalho


The yeastSaccharomyces cerevisiae was entrapped within polyacrylamide gel beads by employing a procedure that uses sodium dodecylsulfate as a detergent to improve the spherical configuration of the beads. The resulting preparation showed a rate of fumarate bioconversion to L-malic acid about 60 times higher than that found for the free cells. Almost all fumarate was converted in 30 min of incubation. The thermal stability of the immobilized cells did not significantly differ from the free cells. An optimal pH of 5.7 was found for the immobilized preparation and no succinic acid was detected as a by-product in the incubation mixture.

Index Entries

L-Malic acid Saccharomyces cerevisiae polyacrylamide gel beads 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Kitahara, K., Fukui, S., and Misawa, M. (1960),J. Gen. Appl. Microbiol. 6(2), 108–116.Google Scholar
  2. 2.
    Chibata, I., Tosa, T., and Sato, T. (1974),Appl. Microbiol. 27, 878–885.Google Scholar
  3. 3.
    Tung-Fu, L. (1982),Radiat. Phys. Chetn. 20(3), 209–214.CrossRefGoogle Scholar
  4. 4.
    Ger Offen. 2,450,137 (Cl. co7cg). 24 Apr. 1975. Japan. Appl. 73 118,832. 22 Oct. 1973. 30 p.Google Scholar
  5. 5.
    Yamamoto, K., Tosa, T., Yamashita, K., and Chibata, I. (1977),Biotechnol. Bioeng. 19(8), 1101–1114.CrossRefGoogle Scholar
  6. 6.
    Yamamoto, K., Tosa, T., Yamashita, K., and Chibata, I. (1976),Eur. J. Appl. Microbiol. Biotechnol. 3, 169–183.Google Scholar
  7. 7.
    Takata, I., Yamamoto, K., Tosa, T., and Chibata, I. (1979),Eur. J. Appl. Microbiol. Biotechnol. 7, 161–172.CrossRefGoogle Scholar
  8. 8.
    Yang, L. and Zhong, L. (1980),Wei Shing Wusuch PaO 20(3), 296–302.Google Scholar
  9. 9.
    Figueiredo, Z. M. B. and Carvalho, L. B., Jr. (1991),Appl. Biochem. Biotechnol. 30, 217–224.CrossRefGoogle Scholar
  10. 10.
    Peleg, Y., Rokem, J. S., Goldberg, I., and Pines, O. (1990),Appl. Environ. Microbiol. 56, 2777–2783.Google Scholar
  11. 11.
    Neufeld, R. J., Peleg, Y., Okem, J. S., Pines, O., and Goldberg, I. (1991),Enzyme Microb. Technol. 13, 991–996.CrossRefGoogle Scholar
  12. 12.
    Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951),J. Biol. Chem. 193, 265–275.Google Scholar

Copyright information

© Humana Press Inc. 1994

Authors and Affiliations

  • Elzabeth A. Oliveira
    • 1
  • Aurelio A. R. Costa
    • 1
  • Zilda M. B. Figueiredo
    • 1
  • Luiz B. Carvalho
    • 1
  1. 1.Laboratorio de Enzimologia, Departamento de Bioquimica and Laboratorio de Imunopatologia Keizo AsamiUniversidade Federal de Pernambuco, Cidade UniversitariaRecife, PEBrazil

Personalised recommendations