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Applied Biochemistry and Biotechnology

, Volume 47, Issue 1, pp 65–72 | Cite as

L-malic acid production by entrappedSaccharomyces cerevisiae into polyacrylamide gel beads

  • Elzabeth A. Oliveira
  • Aurelio A. R. Costa
  • Zilda M. B. Figueiredo
  • Luiz B. Carvalho
Article

Abstract

The yeastSaccharomyces cerevisiae was entrapped within polyacrylamide gel beads by employing a procedure that uses sodium dodecylsulfate as a detergent to improve the spherical configuration of the beads. The resulting preparation showed a rate of fumarate bioconversion to L-malic acid about 60 times higher than that found for the free cells. Almost all fumarate was converted in 30 min of incubation. The thermal stability of the immobilized cells did not significantly differ from the free cells. An optimal pH of 5.7 was found for the immobilized preparation and no succinic acid was detected as a by-product in the incubation mixture.

Index Entries

L-Malic acid Saccharomyces cerevisiae polyacrylamide gel beads 

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Copyright information

© Humana Press Inc. 1994

Authors and Affiliations

  • Elzabeth A. Oliveira
    • 1
  • Aurelio A. R. Costa
    • 1
  • Zilda M. B. Figueiredo
    • 1
  • Luiz B. Carvalho
    • 1
  1. 1.Laboratorio de Enzimologia, Departamento de Bioquimica and Laboratorio de Imunopatologia Keizo AsamiUniversidade Federal de Pernambuco, Cidade UniversitariaRecife, PEBrazil

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