Cell Biophysics

, Volume 6, Issue 2, pp 103–116 | Cite as

Automated analysis of living cells through the quantitative use of automated phase contrast microscopy

  • Francesco Beltrame
  • Bruno Bianco
  • Alessandro Chiabrera


A simplified theory of image formation in phase contrast microscopy is presented. It is shown that the phase shift induced in light (related to the refractive index) by the observed object can be reconstructed, point by point, from the phase-contrast digitally sampled image through an appropriate algorithm. This allows one to make quantitative observations on unstained, living cells.

Index Entries

Phase contrast microscopy, of living cells digital image processing, in phase contrast microscopy optical microscopy, of living cells microscopy, of living cell by phase contrast image processing, in phase contrast microscopy cells, automated phase contrast microscopy of 


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  1. 1.
    Zernike, F. (1935),Z. Tech. Physik. 16, 454.Google Scholar
  2. 2.
    Goodman, J. W. (1968),Introduction to Fourier Optics, McGraw-Hill, New York.Google Scholar
  3. 3.
    Papoulis, A. (1968),System and Transforms with Applications in Optics, McGraw-Hill, New York.Google Scholar
  4. 4.
    Quatieri, T. F. (1981),Proc. IEEE,68, 667.Google Scholar
  5. 5.
    Beltrame, F., Chiabrera, A., Grattarola, M., Guerrini, P., Parodi, G., Ponta, D., Vernazza, G., and Viviani, R. (1980),Proceedings of the 2nd Conference of the IEEE EMBS Society, p. 58, IEEE, New York.Google Scholar
  6. 6.
    Beltrame, F., Bianco, B., and Chiabrera A. (1983),Proceedings of the IEEE 71(2), 270.CrossRefGoogle Scholar
  7. 7.
    Beltrame, F., Bianco, B., and Chiabrera, A. (1981), Quantitative Analysis of Phase Contrast Imaging in View of Cytometric Applications,Proceedings of the First European Workshop on Automated Cytology, E. Reinhardt, Stuttgart.Google Scholar

Copyright information

© The Humana Press Inc. 1984

Authors and Affiliations

  • Francesco Beltrame
    • 1
  • Bruno Bianco
    • 1
  • Alessandro Chiabrera
    • 1
  1. 1.Dipartimento di Ingegneria Biofisica ed Elettronica dell ’Università di GenovaGenovaItaly

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