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International Journal of Pancreatology

, Volume 24, Issue 2, pp 77–83 | Cite as

The effects of neutrophil depletion on a completely noninvasive model of acute pancreatitis-associated lung injury

  • Madhav Bhatia
  • Ashok K. Saluja
  • Bernd Hofbauer
  • Hong -Sik Lee
  • Jean -Louis Frossard
  • Michael L. Steer
Original Articles
  • 111 Downloads

Summary

Conclusion

A completely noninvasive animal model of acute pancreatitis-associated lung injury was used to show that neutrophils, activated by pancreatitis, play a key role in mediating pancreatitis-associated lung injury.

Background

Significant pulmonary complications have been known to occur in over 50% of patients with severe acute pancreatitis. Recent studies using a variety of animal models of pancreatitis have suggested that neutrophil activation may play an important role in mediating lung injury. However, in these models, the interpretation of the results is complicated because surgical manipulations alone could have resulted in the activation of neutrophils.

Methods

Young female mice were fed a choline deficient ethionine (CDE) supplemented diet. The severity of pancreatitis was evaluated by measuring hyperamylasemia, acinar cell necrosis, and pancreatic myeloperoxidase activity. Lung injury was quantified by measuring lung microvascular permeability and lung myeloperoxidase activity. To evaluate the role of neutrophils in CDE diet-induced pancreatitis-associated lung injury, animals were pretreated with antineutrophil serum.

Results

Mice fed the CDE diet develop pancreatitis-associated lung injury. Pretreatment of mice with antineutrophil serum results in marked depletion of circulating neutrophils. Under these conditions, the severity of pancreatitis is reduced and lung injury is completely prevented.

Key Words

CDE diet myeloperoxidase acute pancreatitis lung injury neutrophil sequestration 

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Copyright information

© Humana Press Inc. 1998

Authors and Affiliations

  • Madhav Bhatia
    • 1
    • 2
  • Ashok K. Saluja
    • 1
    • 2
  • Bernd Hofbauer
    • 1
    • 2
  • Hong -Sik Lee
    • 1
    • 2
  • Jean -Louis Frossard
    • 1
    • 2
  • Michael L. Steer
    • 1
    • 2
  1. 1.Department of SurgeryBeth Israel Deaconess Medical CenterBoston
  2. 2.Harvard Medical SchoolBoston

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