The effects of neutrophil depletion on a completely noninvasive model of acute pancreatitis-associated lung injury
- 106 Downloads
A completely noninvasive animal model of acute pancreatitis-associated lung injury was used to show that neutrophils, activated by pancreatitis, play a key role in mediating pancreatitis-associated lung injury.
Significant pulmonary complications have been known to occur in over 50% of patients with severe acute pancreatitis. Recent studies using a variety of animal models of pancreatitis have suggested that neutrophil activation may play an important role in mediating lung injury. However, in these models, the interpretation of the results is complicated because surgical manipulations alone could have resulted in the activation of neutrophils.
Young female mice were fed a choline deficient ethionine (CDE) supplemented diet. The severity of pancreatitis was evaluated by measuring hyperamylasemia, acinar cell necrosis, and pancreatic myeloperoxidase activity. Lung injury was quantified by measuring lung microvascular permeability and lung myeloperoxidase activity. To evaluate the role of neutrophils in CDE diet-induced pancreatitis-associated lung injury, animals were pretreated with antineutrophil serum.
Mice fed the CDE diet develop pancreatitis-associated lung injury. Pretreatment of mice with antineutrophil serum results in marked depletion of circulating neutrophils. Under these conditions, the severity of pancreatitis is reduced and lung injury is completely prevented.
Key WordsCDE diet myeloperoxidase acute pancreatitis lung injury neutrophil sequestration
Unable to display preview. Download preview PDF.
- 2.Fedderson CO, Willemer S, Karges W, Puchner A, Adler G, Wichert PV. Acute lung injury in acute experimental pancreatitis in rats. II. Functional studies.Int J Pancreatol 1991; 8: 323–331.Google Scholar
- 5.Yamanaka K, Saluja AK, Brown GE, Hofbauer B, Steer, ML. Protective effects of prostaglandin E1 on acute lung injury of caerulein-induced acute pancreatitis in rats.Amer J Physiol 1996; 272: G23-G30.Google Scholar
- 13.Pierre KJ, Tung KK, Nadj H. A new enzymatic kinetic method for the determination of alpha amylase.Clin Chem 1976; 22: 1219.Google Scholar