Applied Biochemistry and Biotechnology

, Volume 49, Issue 2, pp 165–172 | Cite as

Safe disposal of diisopropyl fluorophosphate (DFP)

  • George Lunn
  • Eric B. Sansone


Diisopropyl fluorophosphate (DFP), a volatile highly toxic enzyme inhibitor, in buffer (pH 3, pH 5, pH 7, pH 9, pH 11, Hank’s, Dulbecco’s, PBS, TBE, and HEPES) or water (10 mM), in DMF solution (200 mM), and bulk quantities can be degraded by adding 1M NaOH. The DFP was completely degraded, as determined by enzymatic assay, and the final reaction mixtures were not mutagenic.

Index entries

Safety degradation stability enzyme inhibitor diisopropyl fluorophosphate dyflos Isoflurophate 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Cohen, J. A., Oosterbaan, R. A., and Berends, F. (1967), inMethods in Enzymology, vol. 11, Hirs, C. H. W., ed., Academic, New York, pp. 686–702.Google Scholar
  2. 2.
    Saunders, B. C. and Stacey, G. J. (1948),J. Chem. Soc, 695–699.Google Scholar
  3. 3.
    Caughey, G. H., Viro, N. F., Lazarus, S. C, and Nadel, J. A. (1988),Biochim. Biophys. Acta 952, 142–149.Google Scholar
  4. 4.
    Park, J. H., Lee, Y. S., Chung, C. H., and Goldberg, A. L. (1988),J. Bacteriol. 170, 921–926.Google Scholar
  5. 5.
    Zanglis, A. and Lianos, E. A. (1987),J. Lab. Clin. Med. 110, 330–337.Google Scholar
  6. 6.
    Fried, V. A., Smith, H. T., Hildebrandt, E., and Weiner, K. (1987),Proc. Natl. Acad. Sci. USA 84, 3685–3689.CrossRefGoogle Scholar
  7. 7.
    Schwartz, L. B. and Bradford, T. R. (1986),J. Biol. Chem. 261, 7372–7379.Google Scholar
  8. 8.
    Cohen, M. L., Geary, L. E., and Wiley, K. S. (1983),J. Pharmacol. Exp. Ther. 224, 379–385.Google Scholar
  9. 9.
    Arky, R., ed. (1994),Physicians’ Desk Reference, 48th ed., Medical Economics Data, Montvale, NJ, pp. 1457,1458.Google Scholar
  10. 10.
    Wilson, B. W. and Walker, C. R. (1974),Proc. Natl. Acad. Sci. USA 71, 3194–3198.CrossRefGoogle Scholar
  11. 11.
    Gordon, C. J. and MacPhail, R. C. (1993),J. Toxicol. Envir. Health 38, 257–271.CrossRefGoogle Scholar
  12. 12.
    Epstein, J. (1970),Science 170, 1396–1398.CrossRefGoogle Scholar
  13. 13.
    Epstein, J. (1974),J. Am. Water Works Assoc. 66, 31–37.Google Scholar
  14. 14.
    Small, M. J. (1984),Compounds Formed from the Chemical Decontamination of HD, GB, and VX and Their Environmental Fate (USAMBRDL-TR-8304; ADA 149515), US Army Medical Research and Development Command, Fort Detrick, Frederick, MD.Google Scholar
  15. 15.
    Yang, Y.C., Baker, J. A., and Ward, J. R. (1992),Chan. Rev. 92, 1729–1743.Google Scholar
  16. 16.
    Ryan, J. A., McGaughran, W. R., Lindemann, C. J., and Zacchei, A. G. (1979),J. Pharm. Sci. 68, 1194,1195.CrossRefGoogle Scholar
  17. 17.
    Anon. (1986),Diisopropyl Fluorophosphate, Technical Information Bulletin Number AL-122, Aldrich Chemical Co., Milwaukee, WI.Google Scholar
  18. 18.
    Hummel, B. C. W. (1959),Can. J. Biochem. Physiol. 37, 1393–1399.Google Scholar
  19. 19.
    Rao, K. N. and Lombardi, B. (1975),Anal. Biochem. 65, 548–551.CrossRefGoogle Scholar
  20. 20.
    Lunn, G. and Sansone, E. B. (1989),J. Chem. Educ. 66, 443–445.CrossRefGoogle Scholar
  21. 21.
    Lunn, G., Sansone, E. B., and Andrews, A. W. (1991),Environ. Molec. Mutagen. 17, 59–62.CrossRefGoogle Scholar
  22. 22.
    Beyer, W. H., ed. (1968),Handbook of Tables for Probability and Statistics, 2nd ed., Chemical Rubber Co., Cleveland, OH, pp. 339–345.Google Scholar
  23. 23.
    Ellin, R. I., Groff, W. A., and Kaminskis, A. (1981),J. Environ. Sci. Health B16, 713–717.Google Scholar

Copyright information

© Humana Press Inc 1994

Authors and Affiliations

  • George Lunn
    • 1
  • Eric B. Sansone
    • 1
  1. 1.NCI-Frederick Cancer Research and Development CenterProgram Resources, Inc./DynCorp, Environmental Control and Research ProgramFrederick

Personalised recommendations