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To extract or not to extract in secretin radioimmunoassay?

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Summary

The importance of an ethanol extraction procedure in the radioimmunoassay of plasma secretin was investigated. The extraction step led to a higher assay sensitivity of 0.35 fmol/ml, compared to 5.93 fmol/ml using unextracted samples. The rise of plasma secretin after infusion of a low dose of secretin (1 pmol·kg−1·h−1) in 10 healthy humans was only detected after sample extraction. Higher doses (3 and 9 pmol·kg−1·h−1) resulted in increments of plasma IRS (immunoreactive secretin), which could be recorded both with and without sample extraction. After a steak meal 7 of 10 subjects showed a significant increase of plasma secretin assaying extracted plasma samples. The secretin release occurred in spikes. The mean increase of plasma IRS in this group was 0.6 fmol/ml, the mean maximal secretin release above basal was 2.3 fmol/ml. Without sample extraction, plasma secretin was not significantly changed. We conclude that plasma samples should be extracted in order to detect physiological postprandial secretin release.

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Fried, M., Schulthess, C., Beglinger, C. et al. To extract or not to extract in secretin radioimmunoassay?. Int J Pancreatol 3, 357–366 (1988). https://doi.org/10.1007/BF02788469

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  • DOI: https://doi.org/10.1007/BF02788469

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