Small bowel bypass prevents the trophic action of cholecystokinin on the rat pancreas
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The effect of a chronic administration of cholecystokinin (CCK) on the rat pancreas has been studied in rats subjected to a 90% jejunoileal bypass or an intestinal transection (controls). Jejunoileal bypass, when compared to transection, did not modify the size of the pancreas but decreased its enzyme content, especially for amylase, and reduced the number of zymogen granules. These structural and biochemical changes were maintained when bypassed animals were treated three times daily and for six days with cholecystokinin (20 Ivy Dog Units (IDU)/kg). In contrast, CCK treatment in transected animals induced growth of the pancreas due to cellular hypertrophy and hyperplasia; pancreatic enzyme content, especially for chymotrypsin, and the population of zymogen granules in acinar cells were also enhanced. It is concluded that jejunoileal bypass prevents the trophic action of chronic CCK on the pancreas.
Key wordsPancreas Growth Cholecystokinin Intestinal bypass Rat
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- 4.Booth, A.N., Robbins, D.J., Ribelin, W.E. and de Eds, F., Effect of raw soybean meal and amino acids on pancreatic hypertrophy in rats, Proc. Soc. Exp. Biol. Med., 1960; 104: 681–683.Google Scholar
- 5.Miazza, B.M., Levan van Hung, Vaja, S. and Dowling, R.H., Pancreatic hyperplasia after pancreatico-biliary diversion (PBD) in orally and parenterally fed rats. In: J.W.L. Robinson, R.H. Dowling and E.D. Riecken (Eds.), Mechanisms of Intestinal Adaptation, MTP Press, Lancaster, 1982; 481–492.Google Scholar
- 7.Levan, V.H., Liddle, R.A. and Green G.M.: Effect of trypsin and luminal nutrients in bypassed intestine on plasma CCK and pancreatic growth in rats with jejunal bypass, Third Int. Conf. on Intestinal and Pancreatic Adaptation, Titisee, F.R.G., 1986 (abstract).Google Scholar
- 8.Mayston, P.D. and Barrowman, J.A., The influence of chronic administration of pentagastrin on the rat pancreas, Q. J. Exp. Physiol., 1971; 56: 113–122.Google Scholar
- 14.Barrowman, J.A. and Mayston, P.D., The trophic influence of cholecystokinin on the rat pancreas, J. Physiol., 1974; 238: 73–75P.Google Scholar
- 17.Temler, R.S., Dormond, C.A., Simon, E. and Morel, B., The effect on rat pancreas of feeding Kunitz Soya bean trypsin inhibitor or of repeated injections of purified cholecystokinin, Int. J. Vit. Nutr. Res., 1982; 52 (2): 223.Google Scholar
- 18.Ihse, I., Arnesjo, B. and Lundquist, I., Effects on exocrine and endocrine rat pancreas of long-term administration of CCK-PZ (cholecystokinin-pancreozymin) or synthetic octapeptide-CCK-PZ, Scand. J. Gastroenterol., 1976; 11: 529–535.Google Scholar
- 20.Morisset, J., Chamberland, S., Gilbert, L., Lord, A. and Larose, L., Study of pancreatic DNA synthesis in vivo and in vitro following caerulein treatment in vivo, Biomed. Res., 1982; 3, (2): 151–158.Google Scholar
- 24.Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J., Protein measurement with Folin phenol reagent, J. Biol. Chem., 1951; 193: 262–275.Google Scholar
- 26.Schneider, W.C., Determination of nucleic acids in tissues by pentose analysis. In: S.P. Colowick and N.O. Kaplan (Eds.), Methods in Enzymology, Academic Press, New York, 1957; 680–684.Google Scholar
- 30.Burhol, P.G., Jenssen, T.G., Jorde, R., Lygren, I. and Johnson, J.A., Plasma cholecystokinin (CCK) before and after jejunoileal bypass operation in obese patients with reference to appetite regulation, Int. J. Obesity, 1984; 8: 233–236.Google Scholar