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Alterations in EGF binding to acini during pancreatic regeneration in the rat

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Summary

The binding of125I-labeled epidermal growth factor (EGF) was compared in acini isolated from the regenerating remnant following 90% partial pancreatectomy (ppx) and from the pancreas of sham-pancreatectomized (sham-ppx) rats. Saturation binding studies with increasing amounts of unlabeled EGF revealed that cell-associated radioactivity was decreased in acini from the regenerating remnant by comparison to acini from sham-ppx rats. Analysis of these data indicated that binding was decreased by 35% and 27% at 3 and 7 days post-ppx, respectively. This alteration in EGF binding coincides with increased exocrine cell mitotic activity. EGF binding was normalized at 14 days post-ppx, at which time the exocrine cell mitotic activity is no longer increased (Brockenbrough et al. 1987, Diabetes).125I-insulin binding was the same in ppx and sham-ppx acini at 3 days post-ppx. Furthermore, plasma EGF concentrations were the same in ppx and sham-ppx rats. These data indicate that EGF handling by the pancreatic acinar cell is altered during the proliferative response to ppx.

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Authors and Affiliations

  1. Dept. of Medicine, Endocrinology Division, University of Arizona Health Sciences Center, 1501 N. Campbell Ave., 85724, Tucson, AZ, U.S.A.

    J. Scott Brockenbrough & Murray Korc

  2. Joslin Diabetes Center, 02215, Boston, MA

    Gordon C. Weir

  3. Department of Physiology, Medical College of Virginia, 23298, Richmond, VA, U.S.A.

    J. Scott Brockenbrough & Murray Korc

  4. Endocrine Division, Department of Medicine, Medical College of Virginia, 23298, Richmond, VA, U.S.A.

    Gordon C. Weir

Authors
  1. J. Scott Brockenbrough
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  2. Gordon C. Weir
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  3. Murray Korc
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Brockenbrough, J.S., Weir, G.C. & Korc, M. Alterations in EGF binding to acini during pancreatic regeneration in the rat. Int J Pancreatol 3, 415–424 (1988). https://doi.org/10.1007/BF02788200

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  • DOI: https://doi.org/10.1007/BF02788200

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