Abstract
The stability of invertase was studied under various conditions, including at 75°C, in presence of stabilizers (sorbitol and glycerol) at 75°C, and in the presence of denaturants (urea and trichloroacetic acid) at 37°C in reverse micelles. Stability of the invertase in reverse micelles was found to be improved over that of the enzyme in bulk aqueous solution. Sorbitol could enhance enzyme stability as it does in the bulk aqueous system. The stabilizing effect of glycerol was reduced in reverse micelles. The denaturation pattern of urea remains unaltered. However, the denaturation effect of trichloroacetic acid has been reduced in reverse micelles.
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Subramani, S., Shah, C. & Madamwar, D. Stability of invertase in reverse micelles. Appl Biochem Biotechnol 60, 33–39 (1996). https://doi.org/10.1007/BF02788057
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DOI: https://doi.org/10.1007/BF02788057