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Applied Biochemistry and Biotechnology

, Volume 42, Issue 2–3, pp 207–219 | Cite as

Immunological detection of glycoproteins on blots based on labeling with digoxigenin

  • Anton Haselbeck
  • Wolfgang Hösel
Protocols in Biotechnology

Abstract

In the following, we describe the application of the DIG/antiDIG system for the (structural) analysis of glycoproteins on blots. Special emphasis is being placed on the variety of the different DIG applications in order to obtain structural information concerning the glycoprotein carbohydrate chains. Prominent among them is the use of lectins with well-known specificities for carbohydrate structures.

Index Entries

Glycoproteins digoxigenin immunological detection 

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Copyright information

© Humana Press Inc. 1993

Authors and Affiliations

  • Anton Haselbeck
    • 1
  • Wolfgang Hösel
    • 1
  1. 1.Department of BiochemistryR&D BiotechnologyPenzberg/OBBGermany

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