Abstract
In the following, we describe the application of the DIG/antiDIG system for the (structural) analysis of glycoproteins on blots. Special emphasis is being placed on the variety of the different DIG applications in order to obtain structural information concerning the glycoprotein carbohydrate chains. Prominent among them is the use of lectins with well-known specificities for carbohydrate structures.
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This article first appeared in: Hounsell, E. F., ed. (1993),Methods in Molecular Biollogy, vol. 14: Glycoprotein Analysis in Biomedicine, Humana Press Inc., Totowa, NJ.
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Haselbeck, A., Hösel, W. Immunological detection of glycoproteins on blots based on labeling with digoxigenin. Appl Biochem Biotechnol 42, 207–219 (1993). https://doi.org/10.1007/BF02788054
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DOI: https://doi.org/10.1007/BF02788054