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Applied Biochemistry and Biotechnology

, Volume 42, Issue 2–3, pp 201–206 | Cite as

Polyacrylamide gel electrophoresis of proteoglycans in large-pore gradient gels

  • Vladimír Vilím
Protocols in Biotechnology

Abstract

A procedure is described for preparing large-pore polyacrylamide gradient gels (1.2–16% T, 10–4% C) that allow the separation of large proteoglycans of molecular mass up to several millions.

Index Entries

Polyacrylamide gel electrophoresis proteoglycans high-molecular-mass proteins 

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References

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    Laemmli, U. K. (1970),Nature 227, 680–685.CrossRefGoogle Scholar
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    Vilim, V. and Krajickova, J. (1991),Anal. Biochem. 196, 34–39.CrossRefGoogle Scholar
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    Fisher, L. W., Termine, J. D., Dejter, S. W., Jr., Whitson, S. W., Yanagishita, M., Kimura, J. H., Hascall, V. C., Kleinman, H. K., Hasell, J. R., and Nüsson, B. (1983),J. Biol. Chem. 258, 6588–6594.Google Scholar

Copyright information

© Humana Press Inc. 1993

Authors and Affiliations

  • Vladimír Vilím
    • 1
  1. 1.Institute of RheumatologyPraha 2Czech Republic

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