Applied Biochemistry and Biotechnology

, Volume 42, Issue 2–3, pp 201–206 | Cite as

Polyacrylamide gel electrophoresis of proteoglycans in large-pore gradient gels

  • Vladimír Vilím
Protocols in Biotechnology


A procedure is described for preparing large-pore polyacrylamide gradient gels (1.2–16% T, 10–4% C) that allow the separation of large proteoglycans of molecular mass up to several millions.

Index Entries

Polyacrylamide gel electrophoresis proteoglycans high-molecular-mass proteins 


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Copyright information

© Humana Press Inc. 1993

Authors and Affiliations

  • Vladimír Vilím
    • 1
  1. 1.Institute of RheumatologyPraha 2Czech Republic

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