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Applied Biochemistry and Biotechnology

, Volume 42, Issue 2–3, pp 181–188 | Cite as

Silver staining of DNA in polyacrylamide gels

  • Brant J. Bassam
  • Gustavo Caetano-Anollés
Protocols in Biotechnology

Abstract

Nucleic acids can be detected at the picogram level using a quick and simple silver staining method (2). Using very thin polyesterbacked polyacrylamide gels, a further simplified protocol was compared to other widely used silver staining procedures. The improved protocol described here was the most sensitive, the fastest to perform, and had relatively few steps and reagents. This method also produced the least number of staining artifacts and offered images of high contrast.

Index Entries

DNA polyacrylamide gels silver staining 

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References

  1. 1.
    Bassam, B. J., Caetano-Anollés, G., and Gresshoff, P. M. (1991),Anal. Biochem. 80, 81–84.Google Scholar
  2. 2.
    Allen, R. C., Graves, G., and Budowle, B. (1989),BioTechniques 7, 736–744.Google Scholar
  3. 3.
    Budowle, B., Chakraborty, R., Guisti, A. M., Eisenberg, A. J., and Allen, R. C. (1991),Am. J. Hum. Genet. 48, 137–144.Google Scholar
  4. 4.
    Caetano-Anollés, G., Bassam, B. J., and Gresshoff, P. M. (1991),Bio/Technology 9, 553–557.CrossRefGoogle Scholar
  5. 5.
    Rabilloud, T. (1990),Electrophoresis 11, 785–794.CrossRefGoogle Scholar
  6. 6.
    Merril, C. R. (1990),Meth. Enzymol. 182, 477–488.CrossRefGoogle Scholar
  7. 7.
    Goldman, D. and Merril, C. R. (1982),Electrophoresis 3, 24–26.CrossRefGoogle Scholar
  8. 8.
    Gottlieb, M. and Chavco, K. (1987),Anal. Biochem. 165, 33–37.CrossRefGoogle Scholar
  9. 9.
    Caetano-Anollés, G., Bassam, B. J., and Gresshoff, P. M. (1992),Mol. Gen. Genet. 235, 157–165.CrossRefGoogle Scholar
  10. 10.
    Heukeshoven, J. and Dernick, R. (1985),Electrophoresis 6, 103–112.CrossRefGoogle Scholar
  11. 11.
    Wray, W., Boulikas, T., Wray, V. P., and Hancock, R. (1981),Anal. Biochem. 118, 197–203.CrossRefGoogle Scholar
  12. 12.
    Switzer, R. C., III, Merril, C. R., and Shifrin, S. (1979),Anal. Biochem. 98, 231–237.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 1993

Authors and Affiliations

  • Brant J. Bassam
    • 1
  • Gustavo Caetano-Anollés
    • 1
  1. 1.Plant Molecular Genetics, Institute of Agriculture and Center for Legume ResearchThe University of TennesseeKnoxuille

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