Abstract
Nucleic acids can be detected at the picogram level using a quick and simple silver staining method (2). Using very thin polyesterbacked polyacrylamide gels, a further simplified protocol was compared to other widely used silver staining procedures. The improved protocol described here was the most sensitive, the fastest to perform, and had relatively few steps and reagents. This method also produced the least number of staining artifacts and offered images of high contrast.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bassam, B. J., Caetano-Anollés, G., and Gresshoff, P. M. (1991),Anal. Biochem. 80, 81–84.
Allen, R. C., Graves, G., and Budowle, B. (1989),BioTechniques 7, 736–744.
Budowle, B., Chakraborty, R., Guisti, A. M., Eisenberg, A. J., and Allen, R. C. (1991),Am. J. Hum. Genet. 48, 137–144.
Caetano-Anollés, G., Bassam, B. J., and Gresshoff, P. M. (1991),Bio/Technology 9, 553–557.
Rabilloud, T. (1990),Electrophoresis 11, 785–794.
Merril, C. R. (1990),Meth. Enzymol. 182, 477–488.
Goldman, D. and Merril, C. R. (1982),Electrophoresis 3, 24–26.
Gottlieb, M. and Chavco, K. (1987),Anal. Biochem. 165, 33–37.
Caetano-Anollés, G., Bassam, B. J., and Gresshoff, P. M. (1992),Mol. Gen. Genet. 235, 157–165.
Heukeshoven, J. and Dernick, R. (1985),Electrophoresis 6, 103–112.
Wray, W., Boulikas, T., Wray, V. P., and Hancock, R. (1981),Anal. Biochem. 118, 197–203.
Switzer, R. C., III, Merril, C. R., and Shifrin, S. (1979),Anal. Biochem. 98, 231–237.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bassam, B.J., Caetano-Anollés, G. Silver staining of DNA in polyacrylamide gels. Appl Biochem Biotechnol 42, 181–188 (1993). https://doi.org/10.1007/BF02788051
Issue Date:
DOI: https://doi.org/10.1007/BF02788051