Abstract
Total synthesis of human Β-endorphin gene has been designed for the expression in bacterial system. Eight individual oligonucleotides corresponding to the Β-endorphin gene were chemically synthesized and joined through the enzyme-catalyzed reaction. The final yield of the 111-nucleotide-long synthetic Β-endorphin gene construct was about 10% of the total oligonucleotide used. The synthetic human Β-endorphin gene was cloned into the bacteriumEscherichia coli, using pUC8 vector and shown to have the correct nucleotide sequences as designed.
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Kim, M.H., Lee, H.J. & Min, K.T. Chemical synthesis and cloning of human Β-endorphin gene inEscherichia coli . Appl Biochem Biotechnol 50, 35–43 (1995). https://doi.org/10.1007/BF02788038
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DOI: https://doi.org/10.1007/BF02788038