Abstract
Fish muscle extracts (Scomberomorus— brasiliensis- carite) were analyzed for their hypoxanthine content using a flow injection system incorporating an immobilized xanthine oxidase bioreactor. The xanthine oxidase was immobilized under mild conditions to a 2-fluoro-1-methylpyridinium Fractogel support. The uric acid produced from the oxidation of hypoxanthine by the immobilized xanthine oxidase at pH 7.0 and 35‡C was monitored at 290 ran. Hypoxanthine concentrations as low as 4.4 Μmol/L can be detected. Up to 30 samples per hour can be analyzed at a flow rate of 1 mL/min, using 150 ΜL sample volumes and a bioreactor dimension of 1.0 cm x 2.0 mm id. Recovery yields were between 92 and 99%. Both within day and between day precisions gave CVs < 5.00% (n = 30). Good correlation (r = 0.998) is obtained when 78 fish samples were analyzed for their hypoxanthine content both by this FI method and a reference HPLC method.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Burt, J., Stroud, G. D., and Jones, N. R. (1969), inFreezing and Irradiation of Fish, Kreuzer, R., ed., Fishing News Books Ltd., London, p. 367.
Jones, N. R., Murray, J., Ligingston, E. I., and Murray, C. K. (1964),J. Sci. Food Agric. 15, 763.
Watanabe, E., Okuma, H., Takahashi, H., Yazawa, S., and Sekimukai, S. (1992),Anal. Chim. Acta 260, 93.
Warthensen, J. J., Waletzko, P. T., and Bunsta, F. F. (1980),J. Agric. Food Chem. 28, 1308.
Ke, P., and Burns, G. B.J. Assoc. Off. Anal. Chem. 66, 444.
Jones, N. R., Murray, J., and Burt, J. R. (1965),J. Food Sci. 30, 971.
Jahns, F. D., Howe, J. L., Coduri, R. J., and Rand, A. G. (1976),Food Technol. 33, 27.
Beuchant, L. R. (1973),J. Agric. Food Chem. 21, 453.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R.J. Biol. Chem. 193, 265.
Narinesingh, D., Jaipersad, D., and Chang-Yen, I. (1988),Anal. Biochem. 172, 89.
Narinesingh, D. and Ngo, T. T. (1987),Biotechnol. Applied Biochem. 9, 450.
Narinesingh, D., Stoute, V. A., Shaama, F., and Ngo, T. T. (1989),J. Mol. Catalysis 54, 147.
De Renzo, E. C. (1956), inAdvances in Enzymology, vol. 17, Nord, F. F., ed., Interscience, NY, pp. 293–328.
Kenndey, J. F. and Cabrai, J. M. S. (1983), inSolid Phase Biochemistry—Analytical and Synthetic Aspects, vol. 66, Scouten, W.H., ed., John Wiley and Sons, NY pp. 253–390.
Karube, I., Matsuoka, H., Suzuki, S., Watanabe, E., and Toyama, K. (1984),J. Agric. Food Chem. 32, 314.
Luong, J. H. T., Mulchandani, A., and Male, K. B. (1989),Anal. Chim. Acta 221, 215.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Balladin, D.A., Narinesingh, D., Stoute, V.A. et al. Immobilization of xanthine oxidase and its use in the quantitation of hypoxanthine in fish muscle tissue extracts using a flow injection method. Appl Biochem Biotechnol 62, 317–328 (1997). https://doi.org/10.1007/BF02788006
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02788006