Abstract
Effects of water content and carbon and nitrogen sources on the production ofL-glutamate oxidase (GOD) by solid state fermentation (SSF) ofStreptomyces sp. N1 were investigated in a 250-mL shake flask. The results show that in the solid medium containing wheat bran 98% (w/w), KCl 0.2% (w/w), and MgCl2 0.2% (w/w), addition of 2.0-mL water per gram solid medium and 0.4% (w/w) (NH4)2SO4 was the best for GOD production. In this work, we also developed a simple technique forin situ measuring oxygen uptake rate (OUR) and carbon dioxide evolution rate (CER) in SSF in a shake flask based on the principle of Warburg manometer. The method was successfully applied to determine OUR and CER values in SSF ofStreptomyces sp. N1. The results indicate that the largest OUR value was detected about one or two days ahead of the highest GOD activity reached depending on the fermentation conditions, and the OUR may be used as anin situ indicator of GOD production in the SSF process.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Kamei, T., Asano, K., Suzuki, H., Matsuzaki, M., and Nakamura, S. (1983) L-glutamate oxidase fromStreptomyces violascene. I. Production, isolation and some properties.Chem. Pharm. Bull. 31, 1307–1314.
Kusakabe, H., Midorikawa, Y., Fujishima, T., Kuninaka, A., and Yoshino, H. (1983),l-glutamate oxidase fromStreptomyces sp. X-119-6 grown on wheat bran.Agric. Biol. Chem. 47, 132–1328.
Ishikawa, H., Misaki, H., and Muto, N. (1986), L-glutamic acid oxidase (H2O2-generating), its production and analytical method therefor. US Patent 4605615.
Bohmer, A., Muller, A., Passarge, M., Liebs, P., Honeck, H., and Muller H. G. (1989), A novel L-glutamate oxidase fromStreptomyces edus. Eur. J. Biochem. 182, 327–332.
Dremel, B., Schmid, R., and Wolfbeis, O. (1991), Comparison of two fibre-optic L-glutamate biosensors based on the detection of oxygen or carbon dioxide, and their application in combination with flow injection analysis to the determination of glutamate.Anal. Chim. Acta 248, 351–359.
Li, Q.-S., Wang, L.-J., and Li, Y.-R. (1996), Screening more producing L-glutamate oxidase strain,Enzyme Microb. Technol.,18, 7–9.
Pandey, A. (1992), Recent process developments in solid-state fermentation,Process Biochem. 27, 109–117.
Umbreit, W. W., Burris, R. H., and Stauffer, J. F. (1972),Manometric and Biochemical Techniques, Minnesota: Burgess, pp. 1–19.
Oriol, E., Raimbault, M., Roussos, S., and Viniegra-Gonzales, G. (1988), Water and water activity in the solid state fermentation of cassava starch byAspergillus niger, Appl. Microbiol. Biotechnol. 27, 498–503.
Ramesh, M. V. and Lonsane, B. K. (1990), Critical importance of moisture content of the medium in alpha-amylase production byBacillus licheniformis M27 in a solid-state fermentation system.Appl. Microbiol. Biotechnol. 33, 501–505.
Desgranges, C., Vergoignan, C., Georges, M., and Durand, A. (1991), Biomass estimation in solid state fermentation,Appl. Microbiol. Biotechnol. 35, 200–205.
Sato, K., Nagatani, M., Nakamura, K., and Sato, S. (1983), Growth estimation ofCandida lipolytica from oxygen uptake in a solid state culture with forced aeration,J. Ferment. Technol. 61, 623–629.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Li, QS., Xu, JJ. & Zhong, JJ. Production ofL-glutamate oxidase andin situ monitoring of oxygen uptake in solid state fermentation ofstreptomyces sp. Nl . Appl Biochem Biotechnol 62, 243–250 (1997). https://doi.org/10.1007/BF02788000
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02788000