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DNA-protein complexes

Natural targets for DNA-hydrolyzing antibodies

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Abstract

Sera of patients with different types of leukemia and acquired immune deficiency syndrome (AIDS) have been examined for the presence of the anti-DNA antibodies. DNA-hydrolyzing activity of antibodies was detected in the sera of patients with chronic lymphoid leukemia (CLL), pre-B-cell acute lymphoid leukemia (pre-B-All), acute myeloleukosis (AML), and AIDS in stages III and IV of the disease. In immunofluorescence tests, the DNA-hydrolyzing antibodies reacted preferentially with proliferating cell nuclei compared with resting cells. A 35-kDa factor was identified as the target for the DNA antibodies in the cell nuclei. The DNA-hydrolyzing antibody fraction from the serum of an AIDS patient crossreacted with HIV I virus proteins gp160, gp120, and p65.

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References

  1. Tramantano, A., Janda, K. D., and Lerner, R. A. (1986),Science 234, 1566.

    Article  Google Scholar 

  2. Shokat, K. M. and Schultz, P. G. (1990),Annu. Rev. Immunol. 8, 335.

    Article  CAS  Google Scholar 

  3. Paul, S., Volle, D. J., Beach, C. M., Johnson, D. R., Powell, M. J., and Massey, R. J. (1989),Science 244, 1158.

    Article  CAS  Google Scholar 

  4. Shuster, A. M., Gololobov, G. V., Kvashuk, O. A., Bogomolova, A. E., Smirnov, I. V., and Gabibov, A. G. (1992),Scienee 256, 665.

    Article  CAS  Google Scholar 

  5. Dubios, E. L. (1976),Lupus Erythematosus, 2nd ed. University of Southern California Press, Los Angeles, CA.

    Google Scholar 

  6. Stollar, B. D. (1988),Int. Rev. Immunol. 5, 1.

    Article  Google Scholar 

  7. Larsen, A. and Weintraub, H. (1982),Cell 29, 609.

    Article  CAS  Google Scholar 

  8. Boyum, A. (1968),Scand. J. Clin. Investigation 21, 77.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Englehardt Institute of Molecular Biology, Russian Academy of Sciences, USSR

    G. V. Gololobov, A. F. Kolesnikov & A. G. Gabibov

  2. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, Ukrainian Academy of Sciences, Ukrain

    S. V. Mikhalap

  3. Institute of Virology, Russian Academy of Medical Sciences, USSR

    A. V. Starov

Authors
  1. G. V. Gololobov
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  2. S. V. Mikhalap
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  3. A. V. Starov
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  4. A. F. Kolesnikov
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  5. A. G. Gabibov
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Gololobov, G.V., Mikhalap, S.V., Starov, A.V. et al. DNA-protein complexes. Appl Biochem Biotechnol 47, 305–315 (1994). https://doi.org/10.1007/BF02787942

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  • DOI: https://doi.org/10.1007/BF02787942

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