Abstract
A simple direct enzyme immunoassay for semiquantitative detec tion of antibodies is suggested. It is based on the difference in diffusion rates in a gel for a synthetic low-mol-wt antigen and of its complexes with antibodies to be detected. Sensitivity and specificity of the devel oped assay are equal to an ELISA method. The assay has been tested with antibodies against HIV protein gp41 in rabbit serum. Possible applications and limitations of the method are discussed.
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References
Lenhoff, H. M. and Ngo, T. T. (1985),Enzyme-Mediated Immunoassay; Plenum, New York.
Gaal, O., Medgeyesi, G. A., and Vereczkey, L. (1980),Electrophoresis in the Separation of Biological Macromolecules, John Wiley, New York.
Osterman, L. A. (1983),Investigation of Biological Macromolecules by Electrofocusing, Immunoelectrofocusing and Radioisitope Methods (in Russ.), Nauka, Moscow.
Bespalova, Zh. D., Pekelis, B. L., Deigin, V. I., Yarova, E. P., Saks, T. R., Ovchinikov, M. V., Efremov, E. E., Sepetov, N. F., Korotkox, A. M., and Molokoedov, A. S. (1990),Collect. Chem. Commun. 55, 2537–2554.
Van Regenmortel, M. H. V. (1988), inSynthetic Polypeptides Antigens, Burdon, R. H. and Van Knippenberg, P. H., eds., Elsevier, Amsterdam
Karulin, A. Yu., Dzantiev, B. V., Zherdev, A. V., Korchagina, I. S., Egorov, A. M., and Berezin, I. V. (1989),Biotechnologiya (in Russian)5, 324–332.
Van Regenmortal, M. H. V. (1988),ISI Atlas of Science: Biochemistry, pp. 30–34.
Van Regenmortel, M. H. V. and de Marcillac, C. D. (1988),Immunol. Lett. 17, 95–108.
Tijssen, P. (1985),Practice and Theory of Enzyme Immunoassays, Elsevier, Amsterdam.
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Semenov, A.N., Efremov, E.E. A simple direct enzyme immunoassay of antibodies based on the differences in diffusion rates in a gel of a synthetic antigen and of its complex with antibodies. Appl Biochem Biotechnol 56, 73–78 (1996). https://doi.org/10.1007/BF02787871
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DOI: https://doi.org/10.1007/BF02787871