Applied Biochemistry and Biotechnology

, Volume 56, Issue 1, pp 49–58 | Cite as

A simple colorimetric assay for muramic acid and lactic acid

  • Kimberley A. C. C. Taylor
Original Articles


The Barker and Summerson method of assaying lactic acid colorimetrically is modified to provide a simple and fast method of measuring lactic acid and other compounds such as muramic acid and glyceraldehyde that will release acetaldehyde on incubation in hot sulfuric acid. The assay can be done with open tubes and no more complicated equipment than a spectrophotometer. A further modification allows a relatively specific determination of formaldehyde.

Index Entries

Muramic acid lactic acid glyceraldehyde acetaldehyde formaldehyde colorimetric assay 


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  1. 1.
    Figenschou, D. L. and Marais, J. P. (1991),Anal. Biochem. 195, 308–312.CrossRefGoogle Scholar
  2. 2.
    Hart, S. P. and Doyle, J. J. (1985),J. Anim. Sci. 61, 975–984.Google Scholar
  3. 3.
    Tusseau, D. and Benoit, C. (1987),J. Chromatog. 395, 323–333.CrossRefGoogle Scholar
  4. 4.
    Hongo, M., Nomura, Y., and Iwahara, M. (1986),Appl. Environ. Microbiol. 52, 314–319.Google Scholar
  5. 5.
    Sato, K. and Tanaka, S. (1990),Anal. Chim. Acta 236, 459–462.CrossRefGoogle Scholar
  6. 6.
    Asabe, Y., Kojima, S., Suzuki, M., and Takitani, S. (1977),Anal. Biochem. 79, 73–82.CrossRefGoogle Scholar
  7. 7.
    Teunissen, M. J., Marras, S. A. E., Op den Camp, H. J. M., and Vogels, G. D. (1989),J. Microbiol. Meth. 10, 247–254.CrossRefGoogle Scholar
  8. 8.
    Barker, S. B. and Summerson, W. H. (1941),J. Biol. Chem. 138, 535–554.Google Scholar
  9. 9.
    Moe, A.J. and Carr, S. B. (1985),J. Dairy Sci. 68, 2220–2226.Google Scholar
  10. 10.
    Singh, R., Kamra, D.N., and Jakhmola, R. C. (1985),Anim. Feed. Sci. Technol. 12, 133–139.CrossRefGoogle Scholar
  11. 11.
    Mimura, T. and Romano, J.-C. (1985),Appl. Environ. Microbiol. 50, 229–237.Google Scholar
  12. 12.
    Moriarity, D. J. W. (1977),Oecologia 26, 317–323.CrossRefGoogle Scholar
  13. 13.
    Tipper, D. J. (1968),Biochem. 7, 1441–1449.CrossRefGoogle Scholar
  14. 14.
    Millar, W. N. and Casida, L. E. (1970),Can. J. Microbiol. 16, 299–304.CrossRefGoogle Scholar
  15. 15.
    Markus, R. L. (1950),Arch. Biochem. 29, 159–165.Google Scholar
  16. 16.
    Hadzija, O. (1974),Anal. Biochem. 60, 512–517.CrossRefGoogle Scholar
  17. 17.
    King, J. D. and White, D. C. (1977),Appl. Environ. Microbiol. 33, 777–783.Google Scholar
  18. 18.
    Walker, S. G., Flemming, C. A., Ferris, F. G., Beveridge, T. J., and Bailey, G. W. (1989),Appl. Environ. Microbiol. 55, 2976–2984.Google Scholar
  19. 19.
    Rao, P. and Pattabiraman, T. N. (1989),Anal. Biochem. 181, 18–22.CrossRefGoogle Scholar
  20. 20.
    Rao, P. and Pattabiraman, T. N. (1990),Anal. Biochem. 189, 178–181.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 1996

Authors and Affiliations

  • Kimberley A. C. C. Taylor
    • 1
  1. 1.Department of Animal and Poultry ScienceUniversity of GuelphGuelph OntarioCanada

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