Effects of abiotic inducers on sesquiterpene synthesis in hairy root and cell-suspension cultures ofhyoscyomus muticus

  • ülkü Mehmetoglu
  • Wayne R. Curtis


The effects of hydrogen peroxide (H2O2), salicylic acid (SA), ethanol (EtOH), and methyl jasmonate (MJ) on the lubimin and solavetivone production in hairy root and cell suspension cultures ofHyoscyamus muticus were investigated. H2O2 (0.194–1.94) mM did not show any positive effect of the lubimin and solavetivone production. Lubimin production of cellsuspension cultures increased 50% in the presence of 40 ΜM SA. For hairy root cultures, lubimin and solavetivone production increased 5 and 48%, respectively, in the presence of 4 ΜM SA •EtOH (20 mM) increased the lubimin and solavetivone concentrations 13% for hairy roots suggesting a potential rule for wounding or membrane disruption. MJ (4 ΜM) and 20 mM EtOH increased the lubimin and solavetivone concentrations 25 and 85%, respectively for hairy roots ofH. muticus.

Index Entries

Hyoscyamus muticus lubimin solavetivone cell suspension hairy root hydrogen peroxide salicylic acid ethanol methyl jasmonate. 


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Copyright information

© Humana Press Inc. 1997

Authors and Affiliations

  • ülkü Mehmetoglu
    • 1
  • Wayne R. Curtis
    • 2
  1. 1.Science Faculty, Chemical Engineering DepartmentAnkara UniversityTandoxanTurkey
  2. 2.Chemical Engineering DepartmentPennsylvania State University

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