Abstract
Flow cytometry (FCM) technique was applied to rapid determination of cell number ofLactococcus garvieae. An antiserum againstL. garvieae was prepared and its immunological property was examined. The present antibody would recognize some epitopes ofL. garvieae with a high specificity. The optimum conditions for the FCM assay were as follows: discriminate value, 60; dilution ratio of the antiserum, 1.0 X 104. Calibration curve forL. garvieae cells was linear, in the range of 2.4 X 104-1.5 X 107 cells/mL. The detection ofL. garvieae in cell suspensions contaminated withEscherichia coli was carried out. A good correlation was observed in the range of 20–90% for the mixing ratio ofL. garvieae. One FCM assay could be completed within 2 min, and the total assay time, including the preparation of bacterial sample, was within 3 h.
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Endo, H., Nakayama, J., Ushio, H. et al. Application of flow cytometry for rapid detection ofLactococcus garvieae . Appl Biochem Biotechnol 75, 295–306 (1998). https://doi.org/10.1007/BF02787782
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DOI: https://doi.org/10.1007/BF02787782