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Importance of growth form on production of hybrid antibiotic byStreptomyces lividans TK21 by fed-batch and continuous fermentation

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Abstract

A fermentation strategy, based on the controlled feeding of growthlimiting nutrients in order to maintain metabolic activity for extended periods, has been examined in the case of the production of a hybrid antibiotic by a transformed strain ofStreptomyces lividans TK21. The fed-batch operation did not improve the results obtained with batch operation. Continuous cultures on defined medium showed stable levels of biomass concentration, but antibiotic production ceased when continuous operation was started. The results obtained indicate the critical influence that morphology of the cell aggregates has on metabolic activity. The antibiotic is produced only in culture conditions providing growth in compact mycelial pellets.

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Authors and Affiliations

  1. Departament d’Enginyeria Química, Facultat de Ciències, Universitat Autònoma de Barcelona, 08 193, Bellaterra, Barcelona, Spain

    Montserrat Sarrà, Francesc Gòdia & Carlos Casas Alvero

  2. Institut de Biologia Fonamental and Departament de Bioquímica i Biologia Molecular, Universitat Autónoma de Barcelona, 08193, Bellaterra, Barcelona, Spain

    Josep A. Pérez-Pons

Authors
  1. Montserrat Sarrà
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  2. Josep A. Pérez-Pons
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  3. Francesc Gòdia
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  4. Carlos Casas Alvero
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Sarrà, M., Pérez-Pons, J.A., Gòdia, F. et al. Importance of growth form on production of hybrid antibiotic byStreptomyces lividans TK21 by fed-batch and continuous fermentation. Appl Biochem Biotechnol 75, 235–248 (1998). https://doi.org/10.1007/BF02787777

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  • DOI: https://doi.org/10.1007/BF02787777

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