Applied Biochemistry and Biotechnology

, Volume 75, Issue 2–3, pp 235–248 | Cite as

Importance of growth form on production of hybrid antibiotic byStreptomyces lividans TK21 by fed-batch and continuous fermentation

  • Montserrat Sarrà
  • Josep A. Pérez-Pons
  • Francesc Gòdia
  • Carlos Casas Alvero
Original Ariticle


A fermentation strategy, based on the controlled feeding of growthlimiting nutrients in order to maintain metabolic activity for extended periods, has been examined in the case of the production of a hybrid antibiotic by a transformed strain ofStreptomyces lividans TK21. The fed-batch operation did not improve the results obtained with batch operation. Continuous cultures on defined medium showed stable levels of biomass concentration, but antibiotic production ceased when continuous operation was started. The results obtained indicate the critical influence that morphology of the cell aggregates has on metabolic activity. The antibiotic is produced only in culture conditions providing growth in compact mycelial pellets.

Index Entries

Hybrid antibiotic Streptomyces lividans fed-batch continuous cultivation 


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  1. 1.
    Hopwood, D. A., Malpartida, R, Kieser, H. M., Ikeda, H., Duncan, J., Fujii, I., et al. (1985),Nature 314, 642–644.CrossRefGoogle Scholar
  2. 2.
    Hutchinson, C. R. (1987),App. Biochem. Biotechnol. 16, 169–189.Google Scholar
  3. 3.
    Martin, J. F. and Demain, A. L. (1980),Microbiol. Rev. 44, 230–251.Google Scholar
  4. 4.
    Schüz, T. C., Fiedler, H. P., and Zähner, H. (1993),Appl. Microbiol. Biotechnol. 39, 433–437.CrossRefGoogle Scholar
  5. 5.
    Suzuki, T., Yamane, T., and Shimizu, S. (1987),Appl. Microbiol. Biotechnol. 25, 526–531.CrossRefGoogle Scholar
  6. 6.
    Hege-Trekatis, D., King, R., Wolf, H., and Giles, F. D.(1992),Appl. Microbiol. Biotechnol. 36, 440–445.CrossRefGoogle Scholar
  7. 7.
    Lohr, D., Buschulte, T., and Gilles, E. D. (1989),Appl. Microbiol. Biotechnol. 32, 274–279.CrossRefGoogle Scholar
  8. 8.
    Fernández de Heredia, M. (1992), PhD Thesis, Universidad Complutense de Madrid.Google Scholar
  9. 9.
    Sarrá, M., Redin, L, Ochin, F., Gódia, F., and Casas, C. (1993),Biotechnol. Lett. 15, 559–564.CrossRefGoogle Scholar
  10. 10.
    Hanson, R. S. and Phillips, J. A. (1981), inManual of Methods for General Bacteriology (Gerhardt, P., Murray, R. G. E., Costilow, R. N., Nester, E. W., Wood, W. A., Krieg, N. R., and Phillips, G. B., eds.), American Society for Microbiology. Washington, D.C., pp. 332–362.Google Scholar
  11. 11.
    Hopwood, D. A., Bibb, M. J., Chater, K. F., Kieser, H. M., Lydiate, D. J., Smith, C. P., Ward, J. M., and Schrempf, H. S. (1985),Genetic manipulation of Streptomyces. A laboratory Manual. John Innes Foundation, Norwich.Google Scholar
  12. 12.
    Wrigley-Jones, C. A., Richards, H., Thomas, C. R., and Ward, J. (1993),Biotechnol. Bioeng. 41, 148–155.CrossRefGoogle Scholar
  13. 13.
    Noack, D. (1988),J. Basic Microbiol. 28, 101–106.CrossRefGoogle Scholar
  14. 14.
    Doull, J. L. and Vining, L. C. (1989),FEMS Microbiol. Lett. 65, 265–268.CrossRefGoogle Scholar
  15. 15.
    Doull, J. L. and Vining, L. C. (1990),Appl. Microbiol. Biotechnol. 32, 449–454.CrossRefGoogle Scholar
  16. 16.
    Braun, S. and Vecht-Lifshitz, S. E. (1991),Trends Biotech. 9, 63–68.Google Scholar
  17. 17.
    Kulaev, J. S. (1979),Biochemistry of Inorganic Polyphosphates, Wiley, London.Google Scholar
  18. 18.
    Mundry, C. and Kuhn, K. P.(1991),Appl. Microbiol. Biotechnol. 35, 306–311.CrossRefGoogle Scholar
  19. 19.
    Sarrà, M., Casas, C., and Gòdia, F. (1997),Biotechnol. Bioeng. 53, 601–610.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Montserrat Sarrà
    • 1
  • Josep A. Pérez-Pons
    • 2
  • Francesc Gòdia
    • 1
  • Carlos Casas Alvero
    • 1
  1. 1.Departament d’Enginyeria Química, Facultat de CiènciesUniversitat Autònoma de BarcelonaBellaterra, BarcelonaSpain
  2. 2.Institut de Biologia Fonamental and Departament de Bioquímica i Biologia MolecularUniversitat Autónoma de BarcelonaBellaterra, BarcelonaSpain

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