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Applied Biochemistry and Biotechnology

, Volume 75, Issue 2–3, pp 235–248 | Cite as

Importance of growth form on production of hybrid antibiotic byStreptomyces lividans TK21 by fed-batch and continuous fermentation

  • Montserrat Sarrà
  • Josep A. Pérez-Pons
  • Francesc Gòdia
  • Carlos Casas Alvero
Original Ariticle

Abstract

A fermentation strategy, based on the controlled feeding of growthlimiting nutrients in order to maintain metabolic activity for extended periods, has been examined in the case of the production of a hybrid antibiotic by a transformed strain ofStreptomyces lividans TK21. The fed-batch operation did not improve the results obtained with batch operation. Continuous cultures on defined medium showed stable levels of biomass concentration, but antibiotic production ceased when continuous operation was started. The results obtained indicate the critical influence that morphology of the cell aggregates has on metabolic activity. The antibiotic is produced only in culture conditions providing growth in compact mycelial pellets.

Index Entries

Hybrid antibiotic Streptomyces lividans fed-batch continuous cultivation 

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Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Montserrat Sarrà
    • 1
  • Josep A. Pérez-Pons
    • 2
  • Francesc Gòdia
    • 1
  • Carlos Casas Alvero
    • 1
  1. 1.Departament d’Enginyeria Química, Facultat de CiènciesUniversitat Autònoma de BarcelonaBellaterra, BarcelonaSpain
  2. 2.Institut de Biologia Fonamental and Departament de Bioquímica i Biologia MolecularUniversitat Autónoma de BarcelonaBellaterra, BarcelonaSpain

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