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Applied Biochemistry and Biotechnology

, Volume 75, Issue 1, pp 93–102 | Cite as

Antibody purification methods

  • M. A. Vljayalakshmi
Original Articles

Abstract

Antibodies (Abs) from the sera of patients with autoimmune diseases are reported to have different catalytic functions. Their recovery by effi cient purification methods is, therefore, a crucial step. This article reviews different available methods for their recovery and emphasizes a new approach, namely adsorbents with immobilized histidine, which allows a good purification both in yield and purity of Abs, with the addi tional advantage of using gentle elution conditions. This, in turn, will ensure the recovery of intact (nondenatured) catalytically functional Abs, directly from the sera.

Index Entries

Autoimmune antibodies catalytic antibodies human serum pseudobioaffinity chromatography histidine ligand affinity gentle elution conditions 

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Copyright information

© Humana Press Inc 1998

Authors and Affiliations

  • M. A. Vljayalakshmi
    • 1
  1. 1.Laboratoire d’Interactions Moléculaires et Technologie des Separations, CNRS UPRES A 6022Université de Technologie de CompiegneCompiegneTrance

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