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Antibody purification methods

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Abstract

Antibodies (Abs) from the sera of patients with autoimmune diseases are reported to have different catalytic functions. Their recovery by effi cient purification methods is, therefore, a crucial step. This article reviews different available methods for their recovery and emphasizes a new approach, namely adsorbents with immobilized histidine, which allows a good purification both in yield and purity of Abs, with the addi tional advantage of using gentle elution conditions. This, in turn, will ensure the recovery of intact (nondenatured) catalytically functional Abs, directly from the sera.

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Authors and Affiliations

  1. Laboratoire d’Interactions Moléculaires et Technologie des Separations, CNRS UPRES A 6022, Université de Technologie de Compiegne, B.P.529, 60205, Compiegne, Trance

    M. A. Vljayalakshmi

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  1. M. A. Vljayalakshmi
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Vljayalakshmi, M.A. Antibody purification methods. Appl Biochem Biotechnol 75, 93–102 (1998). https://doi.org/10.1007/BF02787711

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  • DOI: https://doi.org/10.1007/BF02787711

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