International Journal of Pancreatology

, Volume 23, Issue 1, pp 31–39 | Cite as

Pancreatic bicarbonate response to intraduodenal tryptophan in dogs

Role of muscarinic M1-receptors and cholecystokinin
  • Elke Niebergall-Roth
  • Stephan Teyssen
  • Mark Hartel
  • Christoph Beglinger
  • Rudolf L. Riepl
  • Manfred V. Singer
Original Articles



In dogs
  1. 1.

    Activation of cholecystokinin-receptors is needed for an adequate pancreatic bicarbonate response to secretin;

  2. 2.

    Cholinergic nerve fibers ending on M1-receptors are probably of little or no importance for the bicarbonate response to secretin in the given dose;

  3. 3.

    The bicarbonate response to tryptophan, given with a secretin background, is controlled by cholinergic M1-fibers and by cholecystokinin;

  4. 4.

    M1-fibers mainly mediate the bicarbonate response to low loads of tryptophan, whereas cholecystokinin controls the response to low and high loads of tryptophan; and

  5. 5.

    Both mediators interact in a synergistic manner.



In six conscious dogs with chronic gastric and duodenal fistulas, we compared the action of the M1-receptor antagonisttelenzepine (20.25–81.0 nmol/kg/h), the cholecystokinin-receptor antagonist L-364,718 (0.025–0.1 mg/kg/h), and combinations of both on the pancreatic bicarbonate response to graded loads of intraduodenal tryptophan (0.37–10.0 mmol/h), given against a background of secretin (20.5 pmol/kg/h).


Secretin significantly (p<0.05) stimulated the pancreatic bicarbonate output above basal levels. All doses of L-374,718, but not telenzepine, significantly decreased the bicarbonate response to secretin by up to 64%. Additional administration of telenzepine together with L-364,718 had no further inhibitory effect on the secretinstimulated bicarbonate output as compared to L-364,718 given alone. All loads of tryptophan significantly increased the bicarbonate output over that seen with secretin alone (= incremental bicarbonate response to tryptophan). Telenzepine significantly decreased the incremental bicarbonate response to the two lower loads (0.37–1.1 mmol/h) of tryptophan (by 82–124%); L-364,718 decreased the incremental bicarbonate response to all loads of tryptophan (by 50–118%). The incremental bicarbonate output, as well as the 180-min integrated bicarbonate response to all loads of tryptophan, were abolished by all combinations of both antagonists.

Key Words

Cholecystokinin dog L-364,718 M1-receptor pancreatic bicarbonate secretion telenzepine 


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Copyright information

© Humana Press Inc. 1998

Authors and Affiliations

  • Elke Niebergall-Roth
    • 1
  • Stephan Teyssen
    • 1
  • Mark Hartel
    • 2
  • Christoph Beglinger
    • 3
  • Rudolf L. Riepl
    • 4
  • Manfred V. Singer
    • 1
  1. 1.Dept. of Medicine IV (Gastroenterology)University Hospital of Heidelberg at MannheimMannheimGermany
  2. 2.Department of SurgeryUniversity Hospital of Heidelberg at MannheimMannheimGermany
  3. 3.Department of GastroenterologyUniversity Hospital of BaselBaselSwitzerland
  4. 4.Department of Gastroenterology, Klinikum InnenstadtUniversity of MunichMunichGermany

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