Abstract
Cucumisin (EC 3.4.21.25) isolated from prince melon fruit is a plant serine protease. Its milk-clotting activity was compared with plant cysteine proteases such as papain (EC 3.4.22.2) and ficain (EC 3.4.22.3). Cucumisin was more stable than papain under the condition of pH 7.1, 37‡C for 24 h. The milk-clotting activity of cucumisin was the same to that of papain and was half value of that of ficain.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Foltmann, B. (1987), inCheese: Chemistry, Physics and Microbiology, vol. 1, Fox, P. F., ed., Elsevier Applied Science, London, pp. 33–61.
Otani, H., Matsumori, M., and Hosono, A. (1991),Anim. Sci. Technol. 62, 424–432.
Aworh, O. C. and Nakai, S. (1986),J. Food Sci. 51, 1569,1570.
Kaneda, M. and Tominaga, N. (1975),J. Biochem. 78, 1287–1296.
Boller, T. (1986), inPlant Proteolytic Enzymes, vol. 1, Dalling, M. J., ed., CRC, FL, pp. 67–96.
Kaneda, M., Minematsu, Y., Powers, J. C., and Tominaga, N. (1986),Agric. Biol. Chem. 50, 1075,1076.
Kaneda, M., Ohmine, H., Yonezawa, H., and Tominaga, N. (1984),J. Biochem. 95, 825–829.
Kunitz, M. (1947),J. Gen. Physiol. 30, 291–310.
Arima, K., Yu, J., and Iwasaki, S. (1970), inMethods in Enzymology, vol. 19, Perlmann, G. E., and Lorand, L., eds., Academic, NY, pp. 446–459.
Kaneda, M. and Tominaga, N. (1987),Agric. Biol. Chem. 51, 489–492.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Uchikoba, T., Kaneda, M. Milk-clotting activity of cucumisin, a plant serine protease from melon fruit. Appl Biochem Biotechnol 56, 325–330 (1996). https://doi.org/10.1007/BF02786962
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02786962