Applied Biochemistry and Biotechnology

, Volume 56, Issue 2, pp 197–204 | Cite as

Selective recovery of two enzymes fromBacillus subtilis using aliquat 336 reversed micelles

  • Qing-Long Chang
  • Jia-Yong Chen
Original Articles


Selective separation and purification of two enzymes fromBacillus subtilis (α-amylase and neutral protease) by liquid-liquid extraction using Aliquat 336/isooctane reversed micelles were investigated. After a full forward and backward extraction cycle, α-amylase was separated and purified in the stripping solution, and neutral protease was recovered in the raffinate.

Index Entries

Reversed micelles separation α-amylase neutral protease 


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Copyright information

© Humana Press Inc. 1996

Authors and Affiliations

  • Qing-Long Chang
    • 1
  • Jia-Yong Chen
    • 1
  1. 1.Institute of Chemical MetallurgyChinese Academy of SciencesBeijingChina

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