Applied Biochemistry and Biotechnology

, Volume 74, Issue 1, pp 1–12 | Cite as

Covalently immobilized choline oxidase and cholinesterases on a methacrylate copolymer for disposable membrane biosensors

  • Lucio Doretti
  • Paola Gattolin
  • Anna Burla
  • Daniela Ferrara
  • Silvano Lora
  • Giancarlo Palma
Original Articles


Bienzymatic sensors for the determination of esters of choline were prepared by covalent co-immobilization of cholinesterases and choline oxidase on polymer membranes, obtained by radiation-induced copolymerization of 2-hydroxyethyl methacrylate and glycidyl methacrylate at low temperature. Optimization of the covalent attachment of choline oxidase and acetyl-or butyrylcholinesterase to copolymer was explored. The enzyme-modified polymers were applied on platinum electrodes to form amperometric sensors, based on the electrochemical detection of enzymatically developed hydrogen peroxide. Acetyl-, acetylthiobutyryl-, and butyrylthiocholine contents in standard solutions were measured, and linear calibration curves were determined. Temperature and pH effects on the electrochemical response are described.

Index entries

Biosensors acetylcholinesterase butyrylcholinesterase choline oxidase covalent immobilization radiation induced polymerization 


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Copyright information

© Humana Press Inc 1998

Authors and Affiliations

  • Lucio Doretti
    • 1
  • Paola Gattolin
    • 1
  • Anna Burla
    • 1
  • Daniela Ferrara
    • 1
  • Silvano Lora
    • 2
  • Giancarlo Palma
    • 3
  1. 1.Ufficio Sicurezza e Prevenzione del CNRPadovaItaly
  2. 2.Sezione di LegnaroIstituto di Fotochimica e Radiazioni d’Alto Energia del CNRLegnaro (Padova)Italy
  3. 3.Dipartimento di Chimica FisicaUniversità di PodovaPadovaItaly

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