β-Transelimination of citrus pectin catalyzed by penicillium italicum pectin lyase in a membrane reactor
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Continuous β-transelimination of citrus pectin was achieved using pectin lyase [PNL, poly(methoxygalacturonide)lyase, EC 126.96.36.199] purified fromPenicillium italicum and confined in a membrane reactor. Various operational parameters, such as enzyme and substrate concentrations, filtrate flowrate, and reaction volume were optimized. Kinetic studies indicated that β-transelimination of pectin in the continuous reactor occurred following a first-order reaction with respect to substrate concentration for up to 90 min at 1 mg/mL pectin. This time period could be extended up to 120 min when the reactor was fed with 3–4 mg/mL pectin. During the first 50 h of operation, the system was capable of maintaining a viscosity reduction of 55% below the initial value when it was continuously fed with 4 mg/mL pectin.
Index EntriesPectin lyase EC 188.8.131.52 β-transelimination citrus pectin membrane reactor Penicillium italicum
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