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Applied Biochemistry and Biotechnology

, Volume 55, Issue 3, pp 249–259 | Cite as

β-Transelimination of citrus pectin catalyzed by penicillium italicum pectin lyase in a membrane reactor

  • Itziar Alkorta
  • Carlos Garbisu
  • María J. Llama
  • Juan L. Serra
Original Articles

Abstract

Continuous β-transelimination of citrus pectin was achieved using pectin lyase [PNL, poly(methoxygalacturonide)lyase, EC 4.2.2.10] purified fromPenicillium italicum and confined in a membrane reactor. Various operational parameters, such as enzyme and substrate concentrations, filtrate flowrate, and reaction volume were optimized. Kinetic studies indicated that β-transelimination of pectin in the continuous reactor occurred following a first-order reaction with respect to substrate concentration for up to 90 min at 1 mg/mL pectin. This time period could be extended up to 120 min when the reactor was fed with 3–4 mg/mL pectin. During the first 50 h of operation, the system was capable of maintaining a viscosity reduction of 55% below the initial value when it was continuously fed with 4 mg/mL pectin.

Index Entries

Pectin lyase EC 4.2.2.10 β-transelimination citrus pectin membrane reactor Penicillium italicum 

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Copyright information

© Humana Press Inc 1995

Authors and Affiliations

  • Itziar Alkorta
    • 1
  • Carlos Garbisu
    • 1
  • María J. Llama
    • 1
  • Juan L. Serra
    • 1
  1. 1.Departamento de Bioquímica y Biología Molecular, Facultad de CienciasUniversidad del País VascoBilbaoSpain

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