Applied Biochemistry and Biotechnology

, Volume 55, Issue 3, pp 207–218 | Cite as

Superactivity of peroxidase solubilized in reversed micellar systems

  • L. Setti
  • P. Fevereiro
  • E. P. Melo
  • P. G. Pifferi
  • J. M. S. Cabral
  • M. R. Aires-Barros
Original Articles


Vaccinium mirtyllus peroxidase solubilized in reversed micelles was used for the oxidation of guaiacol. Some relevant parameters for the enzymatic activity, such as pH,w o (molar ratio water/surfactant), surfactant type and concentration, and cosurfactant concentration, were investigated. The peroxidase showed higher activities in reversed micelles than in aqueous solution. The stability of the peroxidase in reversed micelles was also studied, namely, the effect ofw o and temperature on enzyme deactivation. The peroxidase displayed higher stabilities in CTAB/hexanol in isooctane reversed micelles, with halflife times higher than 500 h.

Index Entries

Vaccinium mirtyllus peroxidase reversed micelles superactivity stability 


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Copyright information

© Humana Press Inc 1995

Authors and Affiliations

  • L. Setti
    • 1
  • P. Fevereiro
    • 2
  • E. P. Melo
    • 3
  • P. G. Pifferi
    • 1
  • J. M. S. Cabral
    • 3
  • M. R. Aires-Barros
    • 3
  1. 1.Dipartimento di Chimica Industriale e dei MaterialiBolognaItaly
  2. 2.Instituto de Tecnologia e Química BiológicaUniversidade Nova LisboaOeirasPortugal
  3. 3.Laboratório de Engenharia BioquímicaInstituto Superior TécnicoLisboaPortugal

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