Abstract
C-peptide radioimmunoassay (C-peptide RIA) is widely used in determination of pancreatic B-cell secretion activity.125I labeled TyrC-peptide is indispensable in C-peptide RIA kit. Herein we discuss a way of obtaining recombinant Tyr-C-peptide. Arg32Tyr human proinsulin mutant (R32Y-proinsulin) gene was constructed by site-directed mutagenesis and overexpressed inEscherichia coli. Purified R32Y-proinsulin was converted to insulin and Tyr-C-peptide by trypsin and carboxypeptidase B codigestion. Tyr-C-peptide was isolated through reverse-phase HPLC (RP-HPLC) and identified by C-peptide RIA and amino acid analysis.
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Sun, H., Tang, J. & Hu, M. Preparation of Tyr-C-peptide from genetically altered human insulin precursor. Appl Biochem Biotechnol 55, 167–174 (1995). https://doi.org/10.1007/BF02786858
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DOI: https://doi.org/10.1007/BF02786858