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Preparation of Tyr-C-peptide from genetically altered human insulin precursor

  • Huaiyu Sun
  • Jianguo Tang
  • Meihao Hu
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Abstract

C-peptide radioimmunoassay (C-peptide RIA) is widely used in determination of pancreatic B-cell secretion activity.125I labeled TyrC-peptide is indispensable in C-peptide RIA kit. Herein we discuss a way of obtaining recombinant Tyr-C-peptide. Arg32Tyr human proinsulin mutant (R32Y-proinsulin) gene was constructed by site-directed mutagenesis and overexpressed inEscherichia coli. Purified R32Y-proinsulin was converted to insulin and Tyr-C-peptide by trypsin and carboxypeptidase B codigestion. Tyr-C-peptide was isolated through reverse-phase HPLC (RP-HPLC) and identified by C-peptide RIA and amino acid analysis.

Index Entries

Tyr-C-peptide human proinsulin C-peptide RIA 

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Copyright information

© Humana Press Inc 1995

Authors and Affiliations

  • Huaiyu Sun
    • 1
  • Jianguo Tang
    • 1
  • Meihao Hu
    • 1
  1. 1.The Department of Biochemistry and Molecular Biology, College of Life SciencesPeking UniversityBeijingChina

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