Minor and trace elemental determination in the Indian herbal and other medicinal preparations

  • Dhananjay L. Samudralwar
  • Amar N. Garg
Original Articles


Medicinal plants described in the Indian “Ayurvedic” literature viz. Tulsi (Ocimum sanctum), Gulvel (Tinospora cardifolia), bitter Neem (Azadirachta indica), Kanher (Nerium Åndicum), Vekhand (Acorus calamus), and Peacock's feather (ash) were analyzed for minor and trace elements by instrumental neutron activation analysis. The samples and the standards from the National Institute of Standards and Technology, USA and IAEA, Vienna were irradiated for 5 min, 1 h, 5 h, and 10 h with thermal neutrons at a flux of 1012–1013 n cm−2s−1 in APSARA and CIRUS reactor at BARC Bombay. High resolution γ ray spectrometry was performed using a 45 cm3 HPGe detector and a 4096 MCA system. Concentrations of 13 elements were determined. Zinc, manganese, and sodium were significantly higher in Tulsi leaves while zinc is higher in Neem leaves. Peacock's feathers were found to be rich in manganese, iron, copper, and zinc. A high concentration of mercury was also found in the peacock's feather ash. The therapeutic significance in restoring ionic balance is discussed.

Index Entries

Indian medicinal plants INAA trace elements peacock feathers 


  1. 1.
    N. G. Patel, Ayurveda: The Traditional Medicine of India inFolk Medicine, The Art and Science, Chapter 3, R. P. Steiner, ed., American Chemical Society, Washington DC, p 41 (1986).Google Scholar
  2. 2.
    S. N. Parchure,Charak Samhita, vol. 1–13, Sagar publications, Pune, India (1983).Google Scholar
  3. 3.
    A. V. Yadav,Dravyaguna, Part I & II, Baidyanath Publications, Calcutta, pp. 794 (1980).Google Scholar
  4. 4.
    B. Mukerji,The Indian Pharmaceuticals Codex, Indigenous Drugs, vol. 1 Council of Scientific and Industrial Research, New Delhi, pp. 352 (1958).Google Scholar
  5. 5.
    C. R. Bhandari,Vanoshadhi Chandrodaya, vol. 8, The Time Table Press, Banaras, India, pp. 1666 (1925).Google Scholar
  6. 6.
    K. S. Chen, C. L. Tseng, and T. H. Lin, Trace elements in natural drugs determined by INAA,J. Radioanal Nucl. Chem. 170, 265–280 (1993).CrossRefGoogle Scholar
  7. 7.
    G. P. Garg, S. K. Nigam, and C. W. Ogle, The gastric antiulcer effects of the leaves of the neem tree,Planta Med. 59, 215–217 (1993).PubMedCrossRefGoogle Scholar
  8. 8.
    S. S. Riar, C. Devkumar, R. C. Sawney, G. Ilavazhagan, J. Bardhan, A. K. Kain, P. Thomas, R. Singh, B. Singh, and R. Parshad, Antifertility activity of volatile fraction of neem oil,Contraception 44, 319–326 (1991).PubMedCrossRefGoogle Scholar
  9. 9.
    E. B. Thompson, and C. C. Anderson, Cardiovascular effects of Azadirecta indica extract,J. Pharm. Sci. 67, 1476–1478 (1978).PubMedCrossRefGoogle Scholar
  10. 10.
    Chemical and Engineering News (ACS, USA) Science and Technology Department, Natural product may be Alzheimer's therapeutic.71, 35–36 (1993).Google Scholar
  11. 11.
    A. S. Prasad, ed., Zinc and Copper, inTrace Elements in Human Health and Disease vol. 1, Academic, New York (1976).Google Scholar
  12. 12.
    P. U. Rao, Chemical composition and biological evaluation of debittered and defatted neem (Azadirachta indica) seed kernel cake.J. Am. Oil Chem. Soc. 64, 1348–1351 (1987).CrossRefGoogle Scholar
  13. 13.
    N. S. Rajurkar and M. S. Winchurkar, Instrumental neutron activation analysis of some Ayurvedic medicines,Appl. Radiat. Isot. 43, 1515–1516 (1992).CrossRefGoogle Scholar
  14. 14.
    M. K. Wong, P. Tan, and Y. C. Wee, Heavy metals in some Chinese herbal plants,Biol. Trace Elem. Res. 36, 135–142 (1993).PubMedGoogle Scholar
  15. 15.
    C. L. Ndiokwere, Determination of constituent elements in some Nigerian medicinal plants by thermal neutron activation analysis,J. Radioanal Nucl. Chem. Lett.,85, 325–338 (1984).CrossRefGoogle Scholar
  16. 16.
    G. D. Kanias and A. Loukis, Determination and correlation of active constituents and trace elements in the medicinal plant Thymus capitatus Hoffm. and Link, Frezenius Z,Anal. Chem. 327, 355–357 (1987).CrossRefGoogle Scholar
  17. 17.
    N. K. Mumba, Determination of Mg, Al, Si, Cl and K in some medicinal plants by NAA and REA methods, J. Csikal and G. Peto,Radiochem. Radioanal. Lett. 52, 372–382 (1982).Google Scholar
  18. 18.
    S. Amiel, ed.Nondestructive Activation Analysis with Nuclear Reactors and Radioactive Neutron Sources: Studies in Analytical Chemistry, vol. 3, Elsevier, Amsterdam, pp. 369 (1981).Google Scholar
  19. 19.
    H. K. Wankhade, D. L. Samudralwar, and A. N. Garg, Neutron activation analysis of geological and biological samples using short term reactor irradiation and successive counting,J. Radioanal. Nucl. Chem. Lett. 105, 95–106 (1986).CrossRefGoogle Scholar
  20. 20.
    R. G. Weginwar, D. L. Samudralwar, and A. N. Garg, Determination of P in biological samples by thermal neutron activation followed by β counting,J. Radioanal. Nucl. Chem. Articles 133, 317–324 (1989).CrossRefGoogle Scholar
  21. 21.
    National Institute of Standards and Technology (NIST), Spinach Certificate of Analysis 1979.Google Scholar
  22. 22.
    L. Psonicki and A. N. Hanna, Report on intercomparison IAEA/V-10 of the determination of trace elements in Hay Powder, IAEA, Vienna (1985).Google Scholar
  23. 23.
    H. J. M. Bowen, Kale as a Reference Material inBiological Reference Materials: Availability, Uses and Need for Validation of Nutrient Measurement, W. R. Wolf, ed., John Wiley, New York, (1985) 3–18.Google Scholar
  24. 24.
    D. L. Samudralwar, H. K., Wankhade, and A. N. Garg, Multielemental analysis of IAEA intercomparison standard Hay Powder V-10 and some edible plant leaves by INAA,J. Radioanal. Nucl. Chem. Articles 116, 307–315 (1987).CrossRefGoogle Scholar
  25. 25.
    A. Kabata-Pendias and H. Pendias,Trace Elements in Soils and Plants, CRC, Boca Raton, FL, pp. 315 (1991).Google Scholar
  26. 26.
    R. R. Chattopadhyay, S. K. Sarkar, S. Ganguly, C. Medda, and T. K. Basu, Hepatoprotective activity of acimum-sanctum leaf extract against paracetamol induced hepatic damage in rats,Indian J. Pharmacol. 24, 163–165 (1992).Google Scholar
  27. 27.
    S. Khanna, S. R. Gupta, and J. K. Grover, Effect of long term feeding of tulsi ocimum sanctum on reproductive performance of adult albino rats,Indian J. Exptl. Biol. 24, 302–304 (1986).Google Scholar
  28. 28.
    R. J. P. Williams, The biochemistry of zinc,Polyhedron 6, 61–70 (1987).CrossRefGoogle Scholar
  29. 29.
    V. K. Patel and B. H. Venkatakrishna, Folklore therapeutic indigenious plants in periodontal disorders in India (review, experimental and clinical approach),Int. J. Clin. Pharmacol. Ther. Toxicol. 26, 176–184 (1988).PubMedGoogle Scholar
  30. 30.
    O. K. Osubiojo, Multielement analysis of Nigerian chewing sticks by instrumental neutron activation analysis, V. P. Guinn and A. Okunuga,J. Radioanal. Chem. 74, 149–156 (1982).CrossRefGoogle Scholar
  31. 31.
    R. Puls,Mineral Levels in Animal Health, 2nd ed., Sherpa International, Canada, pp. 356 (1994).Google Scholar

Copyright information

© Humana Press Inc 1996

Authors and Affiliations

  • Dhananjay L. Samudralwar
    • 1
    • 2
  • Amar N. Garg
    • 1
  1. 1.Department of ChemistryNagpur UniversityNagpurIndia
  2. 2.Livestock Disease Diagnosis CenterLexington

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