Characterization of novel neopullulanase fromBacillus polymyxa
Bacillus polymyxa CECT 155 produces an extracellular neopullulanase activity that degrades pullulan to panose. This activity was stimulated by the presence of pullulan in the culture, and repressed by glucose. The apparent mol wt determined for the enzyme was 58 kDa. The optimum pH and temperature for neopullulanase activity were pH 6.0 and 50°C, respectively. The enzyme was stable in a pH range of 4.0–8.0, and temperatures up to 60°C. These properties make it suitable for the saccharification processes in the starch industries.
Index EntriesExtracellular enzyme neopullulanase panose pullulan Bacillus polymyxa
Unable to display preview. Download preview PDF.
- 1.Priest, F. G. and Stark, J. R. (1991), inBiotechnology of Amylodextrin Oligosaccharides, Friedman, R. B., ed., American Chemical Society, Washington, D.C., pp. 72–85.Google Scholar
- 4.Sakano, Y., Masuda, N., and Kobayashi, T. (1971),Agricultural Biol. Chem. 35, 971–973.Google Scholar
- 6.Imanaka, T. and Kuriki, T. (1989J. Bacteriol. 171, 369–374.Google Scholar
- 7.Fogarty, W. M. and Kelly, C. T. (1990), inMicrobial Enzymes and Biotechnology, Fogarty, W. M. and Kelly, C. T., eds., Elsevier, Oxford, pp. 72–132.Google Scholar
- 8.Fogarty, W. M. and Kelly, C. T. (1980), inEconomic Microbiology. Microbial Enzymes and Bioconversions, vol. 5, Rose, A. H., ed., Academic, London, pp. 115–170.Google Scholar
- 9.Morgan, F. J., Adams, K. R., and Priest, F. G. (1979J. Appl. Bacteriol. 46, 291–294.Google Scholar
- 10.Castro, G. R., Ducrey-Santopietro, L. M., and Siñeriz, F. (1992),App. Biochem. Biotech. 37, 227–233.Google Scholar
- 14.Rugbjerg, U. (1987), II Meeting on Industrial Application of Enzymes, Barcelona, Spain.Google Scholar
- 15.Hebeda, R. E., Bowles, L. K., and Teague, W. M. (1990),Cereal Foods World 35, 453–457.Google Scholar
- 16.Hebeda, R. E., Bowles, L. K., and Teague, W. M. (1991),Cereal Foods World 36, 619–624.Google Scholar