Skip to main content
SpringerLink
Log in

Characterization of novel neopullulanase fromBacillus polymyxa

  • Original Articles
  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Bacillus polymyxa CECT 155 produces an extracellular neopullulanase activity that degrades pullulan to panose. This activity was stimulated by the presence of pullulan in the culture, and repressed by glucose. The apparent mol wt determined for the enzyme was 58 kDa. The optimum pH and temperature for neopullulanase activity were pH 6.0 and 50°C, respectively. The enzyme was stable in a pH range of 4.0–8.0, and temperatures up to 60°C. These properties make it suitable for the saccharification processes in the starch industries.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Priest, F. G. and Stark, J. R. (1991), inBiotechnology of Amylodextrin Oligosaccharides, Friedman, R. B., ed., American Chemical Society, Washington, D.C., pp. 72–85.

    Google Scholar 

  2. Wallenfels, K., Bender, H., and Rached, J. R. (1966),Biochem. Biophys. Commun. 22, 254–266.

    Article  CAS  Google Scholar 

  3. Schülein, M. B. and Pedersen, H. (1984),Ann. NY Acad. Sci. 434, 271–274.

    Article  Google Scholar 

  4. Sakano, Y., Masuda, N., and Kobayashi, T. (1971),Agricultural Biol. Chem. 35, 971–973.

    CAS  Google Scholar 

  5. Susuki, Y. and Chishiro, M. (1983),Appl. Microbiol. Biotechnol. 17, 24–29.

    Article  Google Scholar 

  6. Imanaka, T. and Kuriki, T. (1989J. Bacteriol. 171, 369–374.

    CAS  Google Scholar 

  7. Fogarty, W. M. and Kelly, C. T. (1990), inMicrobial Enzymes and Biotechnology, Fogarty, W. M. and Kelly, C. T., eds., Elsevier, Oxford, pp. 72–132.

    Google Scholar 

  8. Fogarty, W. M. and Kelly, C. T. (1980), inEconomic Microbiology. Microbial Enzymes and Bioconversions, vol. 5, Rose, A. H., ed., Academic, London, pp. 115–170.

    Google Scholar 

  9. Morgan, F. J., Adams, K. R., and Priest, F. G. (1979J. Appl. Bacteriol. 46, 291–294.

    CAS  Google Scholar 

  10. Castro, G. R., Ducrey-Santopietro, L. M., and Siñeriz, F. (1992),App. Biochem. Biotech. 37, 227–233.

    CAS  Google Scholar 

  11. Norman, B. E. (1982),Starch/Stärke. 34, 340–346.

    Article  CAS  Google Scholar 

  12. Slominska, L. and Maczynski, M. (1985),Starch/Stärke. 37, 386–390.

    Article  CAS  Google Scholar 

  13. Shiraishi, F., Kawakami, K., Yuasa, A., Kojima, T., and Kusunoki, K. (1987),Biotech. Bioeng. 30, 374–380.

    Article  CAS  Google Scholar 

  14. Rugbjerg, U. (1987), II Meeting on Industrial Application of Enzymes, Barcelona, Spain.

  15. Hebeda, R. E., Bowles, L. K., and Teague, W. M. (1990),Cereal Foods World 35, 453–457.

    CAS  Google Scholar 

  16. Hebeda, R. E., Bowles, L. K., and Teague, W. M. (1991),Cereal Foods World 36, 619–624.

    CAS  Google Scholar 

  17. Bernfeld, P. (1955),Methods Enzymol. 1, 149–158.

    Article  CAS  Google Scholar 

  18. Shen, G. J., Srivastava, K. C., Saha, B. C., and Zeikus, J. G. (1990),App. Microbiol. Biotech. 33, 340–344.

    Article  CAS  Google Scholar 

  19. Prieto, J. A., Bort, B. R., Martinez, J., Randez-Gil, F., Buesa, C., and Sanz, P. (1995),Biochem. Cell Biol. 73, 41–49.

    Article  CAS  Google Scholar 

  20. Kennedy, J. F., Cabalda, V. M., and White, C. A. (1988),Trends Biotechnol. 6, 184–188.

    Article  CAS  Google Scholar 

  21. Nigam, P. and Singh, D. (1995),Enzyme Microbial Tech. 17, 770–778.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute de Agroqu’imica y Tecnolog’ia de los Alimentos, Consejo Superior de Investigaciones Cient’ificas, Mailbox 73, 46100, Burjassot, Spain

    Maria Jesus Yebra, Jaime Arroyo, Pascual Sanz & Jose Antonio Prieto

Authors
  1. Maria Jesus Yebra
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jaime Arroyo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Pascual Sanz
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jose Antonio Prieto
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yebra, M.J., Arroyo, J., Sanz, P. et al. Characterization of novel neopullulanase fromBacillus polymyxa . Appl Biochem Biotechnol 68, 113–120 (1997). https://doi.org/10.1007/BF02785984

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02785984

Index Entries

Search

Navigation