Pretreatment of yellow poplar sawdust by pressure cooking in water
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The pretreatment of yellow poplar wood sawdust using liquid water at temperatures above 220°C enhances enzyme hydrolysis. This paper reviews our prior research and describes the laboratory reactor system currently in use for cooking wood sawdust at temperatures ranging from 220 to 260°C. The wood sawdust at a 6–6.6% solid/liquid slurry was treated in a 2 L, 304 SS, Parr reactor with three turbine propeller agitators and a proportional integral derivative (PID) controller, which controlled temperature within ±1°C. Heat-up times to the final temperatures of 220, 240, or 260°C were achieved in 60–70 min. Hold time at the final temperature was less than 1 min. A serpentine cooling coil, through which tap water was circulated at the completion of the run, cooled the reactor’s contents within 3 min after the maximum temperature was attained. A bottoms port, as well as ports in the reactor’s head plate, facilitated sampling of the slurry and measuring the pH, which changes from an initial value of 5 before cooking to a value of approx 3 after cooking. Enzyme hydrolysis gave 80–90% conversion of cellulose in the pretreated wood to glucose. Simultaneous saccharification and fermentation of washed, pretreated lignocellulose gave an ethanol yield that was 55% of theoretical. Untreated wood sawdust gave less than 5% hydrolysis under the same conditions.
Index EntriesWood water pretreatment enzyme hydrolysis fermentation pretreatment cellulose glucose ethanol
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- 2.Foody, P. (July 24,1984), U.S. Patent 4,461,648.Google Scholar
- 3.Haw, J. F., Maciel, G. E., Linden, J. C., and Murphy, V. G. (1985),Holzforschung 39, 99–107.Google Scholar
- 8.van Walsum, G. P., Allen, S. G., Spencer, M. J., Laser, M. S., Antal, M. J., Jr., and Lynd, L. R. (1996),Appl. Biochem. Biotechnol. 57/58, 157–170.Google Scholar
- 9.Ladisch, M. R., Waugh, L., Westgate, P., Kohlmann, K., Hendrickson, R., Yang, Y., and Ladisch, C. (1992), Ladisch, M. R. and Bose, A., eds., American Chemical Society, Washington, DC, pp. 510–518.Google Scholar
- 10.Kohlmann, K. L., Westgate, P. J., Weil, J., and Ladisch, M. R. (1994), SAE 1993 Transactions.Journal of Aerospace Section. 102, 1476–1483.Google Scholar
- 11.Weil, J., M.S.E. Thesis, Purdue University, May, 1993.Google Scholar
- 15.Kohlmann, K. L., Sarikaya, A., Westgate, P. J., Weil, J., Velayudhan, A., Hendrickson, R., and Ladisch, M. R. (1995), Penner, M. and Saddler, J., eds., American Chemical Society, Washington, DC, ACS Symp. Ser. No. 618,237-255.Google Scholar
- 17.NREL, Chemical Analysis and Testing Standard Procedures, Golden, CO (1994).Google Scholar
- 21.Ladisch, M. R. (1989), inBiomass Handbook, Kitani O. and Hall C. W., eds., Gordon and Breach, New York, pp. 434–451.Google Scholar
- 22.Torget, R. and Hsu, T-A. (1994),Appl. Biochem. and Biotechnol. 45/46, 115–123.Google Scholar
- 23.Torget, R., Hatzis, C., Hayward, T. K., Hsu, T-A., Philippidis, G. P. (1996),Appl. Biochem. Biotechnol. 57/58, 85–101.Google Scholar