Skip to main content
SpringerLink
Log in

Interaction of NAD-dependent dehydrogenases with human erythrocyte membranes

Evidence that D-Glyceraldehyde-3-Phosphate Dehydrogenase and Ladate Dehydrogenase Are Catalytically Active in a Membrane-Bound State

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Interaction of D-glyceraldehyde-3-phosphate dehydrogenase (GPDH) and ladate dehydrogenase with human erythrocyte membranes was studied. Under the conditions of low ionic strength, both enzymes bound to the membranes with similar affinities (Kd ≈ 1 μM). The binding was accompanied by complete inhibition of GPDH and by a 65–75% inhibition of ladate dehydrogenase (LDH). Increasing the ionic strength to physiologically meaningful values (0.15M) completely abolished the inactivation of both dehydrogenases in the presence of erythrocyte membranes, but did not preclude their binding. These results suggest that different modes of enzyme-membrane interaction can be realized under the conditions of low and high ionic strength. They also indicate that GPDH and LDH are capable of functioning in a membrane-bound state.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Murthy, S. N. P., Liu, T., Kaul, R. K., Kohler, H., and Steck, T. L. (1981),J. Biol. Chem. 256, 11,203–11,208.

    CAS  Google Scholar 

  2. Masters, C. J. (1984),J. Cell. Biol. 99, 222–225.

    Article  CAS  Google Scholar 

  3. Ryazanov, A. G., Ashmarina, L. I., and Muronetz, V. I. (1989),Eur. J. Biochem. 158, 49–57.

    Google Scholar 

  4. Tsamaloukas, A. G., Kollecker, P., Maretzki, D., and Rappoport, S. (1978),Acta Biol. Med. Gem. 37, 1273–1278.

    CAS  Google Scholar 

  5. Maretzki, D., Groth, J., Tsamaloukas, A. G., Grundel, M., Kruger, S., and Rappoport, S. (1974),FEBS Lett. 39, 83–87.

    Article  CAS  Google Scholar 

  6. Yu, J. and Steck, T. L. (1975),J. Biol. Chem. 250, 9176–9184.

    CAS  Google Scholar 

  7. Tsai, Inn-Ho, Murthy, S. N. P., and Steck, T. L. (1982),J. Biol. Chem. 257, 1438–1442.

    CAS  Google Scholar 

  8. Harrison, M. L., Rathinavelu, P., Arese, P., Geahlen, R. L., and Low, P. S. (1991),J. Biol. Chem. 266, 4106–4111.

    CAS  Google Scholar 

  9. Low, P. S., Rathinavelu, P., and Harrison, M. L. (1993),J. Biol. Chem. 268, 14,627–14,631.

    CAS  Google Scholar 

  10. Muronetz, V. I., Wang, Z.-X., Keith, T. J., Knull, H. R., and Srivastava, D. K. (1994),Arch. Biochem. Biophys. 313, 253–260.

    Article  CAS  Google Scholar 

  11. Szewczuk, A., Wolna, E., Wolny, M., and Baranowski, T. (1961),Acta Biochim. Pol 2, 201–207.

    Google Scholar 

  12. Hill, E. J., Meriwether, B. P., and Park, J. H. (1975),Analyt. Biochem. 63, 175–182.

    Article  CAS  Google Scholar 

  13. Dodge, J. T., Mitchell, C., and Hanahas, D. J. (1963),Arch. Biochem. Biophys. 100, 119–130.

    Article  CAS  Google Scholar 

  14. Bradford, M. M. (1976),Anal. Biochem. 72, 248–254.

    Article  CAS  Google Scholar 

  15. McDaniel, C. F., Kirtley, M. E., and Tanner, M. J. A. (1974),J. Biol. Chem. 249, 6478–6485.

    CAS  Google Scholar 

  16. Wehland, J., Willingham, M. C., and Sandoval, I. V. (1983),J. Cell. Biol. 97, 1467–1475.

    Article  CAS  Google Scholar 

  17. Serrano, L., Avila, J., and Maccioni, R. B. (1984),Biochemistry 23, 4675–4681.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, 119899, Moscow, Russia

    Vladimir I. Muronetz, Natalia A. Shcherbatova & Natalia K. Nagradova

Authors
  1. Vladimir I. Muronetz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Natalia A. Shcherbatova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Natalia K. Nagradova
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Vladimir I. Muronetz.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Muronetz, V.I., Shcherbatova, N.A. & Nagradova, N.K. Interaction of NAD-dependent dehydrogenases with human erythrocyte membranes. Appl Biochem Biotechnol 61, 39–46 (1996). https://doi.org/10.1007/BF02785686

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02785686

Index entries

Search

Navigation