Abstract
In a recent screening for thermophilic bacteria from Azores hot springs, a Bacillus sp strain 3M, exhibiting cellulase-free extracellular xylanolitic activity, was isolated. Further enzyme characterization from liquid cultures grown on birchwood xylan revealed that the endo-l,4-βxylanase retains 100% of activity for at least 3 d at 55°C. At 80°C, it retains 47% of its maximal activity, and the enzyme is still active at 90°C. The optimum pH of the enzyme has a broad pH range, between 6.0 and 7.5, and it is remarkably active for the alkaline region, exhibiting 89% of relative activity at pH 9.O. The enzyme was partially inactivated by different divalent metal ions. Because of its tolerance for high temperature and pH conditions, and the absence of contaminating cellulase activity, the xylanase produced byBacillus sp 3M appears to be attractive for use in the pulp and paper industry. Indeed, the efficiency of the enzyme application to the kraftEucalyptus pulp was studied for bleaching pretreatment, resulting in a moderate increase of pulp bleachability.
Similar content being viewed by others
References
Viikari, L., Kantelinen, A., Sundquist, J., and Linko, M. (1994),FEMS Microbiol. Rev. 13, 335–350.
Khasin, A., Alchanati, L., and Shoham, Y. (1993),Appl. Environ. Microbiol. 59, 1725–1730.
Wong, K. K. Y., Tan, L. U. L., and Saddler, J. N. (1988),Microbiol. Rev. 52, 305–317.
Nakamura, S., Nakai, R., Wakabayashi, K., Ishiguro, Y., Aono, R., and Hokikoshi, K. (1994),Biosci. Biotech. Biochem. 58, 78–81.
Vishniac, W., and Santer, M. (1957),Bacteriol. Rev. 21, 95–213.
Bailey, M. J., Biely, P., and Poutanen, K. (1992),J. Biotechnol. 23, 257–270.
Ghose, T. K. (1987),Pure Appl. Chem. 59, 257–268.
Li, X.-L., Zhuang, Z.-Q., Dean, J. F. D., Ericksson, K.-E., and Ljungdahl, L. G. (1993),Appl. Environ. Microbiol. 59, 3212–3218.
Lowry, O. H., Rosebrough, N. J., Farr, A. C., and Randall, R. J. (1951),J. Biol. Chem. 193, 265–275.
Miller, G. L. (1959),Anal. Chem. 31, 426–428.
Yang, V. W., Zhuang, Z., Elegir, G., and Jeffries, T. W. (1995),J. Ind. Microbiol. 15, 434–441.
Hortling, B., Korhonen, M., Buchert, M., Sundqvist, M., and Viikari, L. (1994),Holzforschung 48, 441–446.
Gliese, Th., Kleemann, S., and Fischer, K. (1996), inBiotechnology in the Pulp and Paper Industry (Srebotnik, E. and Messner, K., eds.), Facultas-Universitatsverlag, Vienna, pp. 63–67.
Senior, D. J., Mayers, P. R., Miller, D., Sutcliffe, R., Tan, L., and Saddler, J. N. (1988),Biotechnol. Lett. 12, 907–912.
Lee, H., Biely, P., Latta, R. K., Barbosa, M. F. S., and Schneider, H. (1996),Appl. Environ. Microbiol. 2, 320–324.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Marques, S., Alves, L., Ribeiro, S. et al. Characterization of a Thermotolerant and Alkalotolerant Xylanase from a Bacillus sp.. Appl Biochem Biotechnol 73, 159–172 (1998). https://doi.org/10.1007/BF02785652
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02785652