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Photosensitization of the yeast phaffia rhodozyma at a low temperature for screening carotenoid hyperproducing mutants

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Abstract

Phaffia rhodozyma strain Ant-1 produces more carotenoids, known as antioxidants, but it was more sensitive to light plus toluidine blue O (TBO), a superoxide producer, than wild strain 67-385 at 20°C. Carotenoid hyper-producing mutants (CHMs), Ant-1 and 2A2N, exhibited decreased activity of superoxide dismutase (SOD) compared to 67-385, and this is in part responsible for hypersensitivity of the mutants to photosensitization. Light plus TBO at 2°C allowed carotenoid hyperproducing mutants to produce higher colony-forming units than the wild-type. Photosensitization with limited cell metabolism by a low temperature, provides an idea of selective conditions for carotenoid hyperproducers ofP. rhodozyma.

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Authors and Affiliations

  1. Department of Biology, 68-370, Massachusetts Institute of Technology, 77 Massachusetts Ave., 02139, Cambridge, MA

    Gil-Hwan An

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  1. Gil-Hwan An
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An, GH. Photosensitization of the yeast phaffia rhodozyma at a low temperature for screening carotenoid hyperproducing mutants. Appl Biochem Biotechnol 66, 263–268 (1997). https://doi.org/10.1007/BF02785592

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  • DOI: https://doi.org/10.1007/BF02785592

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