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Applied Biochemistry and Biotechnology

, Volume 66, Issue 3, pp 249–262 | Cite as

Analysis of biomass cellulose in simultaneous saccharification and fermentation processes

  • Yun-Chin Chung
  • Alan Bakalinsky
  • Michael H. Penner
Original Articles

Abstract

A direct method for determining the cellulose content of biomass residues resulting from simultaneous saccharifiaction and fermentation (SSF) experiment has been developed and evaluated. The method improves on classical cellulose assays by incorporating the enzymatic removal of yeast glucans from the biomass residue prior to acid hydrolysis and subsequent quantification of cellulose-derived glucose. An appropriate cellulasefree, commercially available, yeast-lysing enzyme preparation fromCytophaga was identified. A freeze-drying step was identified as necessary to render the SSF yeast cells susceptible to enzymatic lysis. The method was applied to the analysis of cellulose and yeast-associated glucans in SSF residues from three pretreated feedstocks; hybrid poplar, switchgrass, and cornstover. Cellulose assays employing the lysing-enzyme preparation demonstrated relative errors up to 7.2% when yeast-associated glucans were not removed prior to analysis of SSF residues. Enzymatic lysis of SSF yeast cells may be viewed as a general preparatory procedure to be used prior to subsequent chemical and physical analysis of SSF residues.

Index Entries

Saccharification fermentation yeast cellulose lysing enzyme 

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Copyright information

© Humana Press Inc 1997

Authors and Affiliations

  • Yun-Chin Chung
    • 1
  • Alan Bakalinsky
    • 1
  • Michael H. Penner
    • 1
  1. 1.Department of Food Science and TechnologyOregon State UniversityCorvallis

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