Reversible (G0) and nonreadily reversible (Q) noncycling cells in human peripheral blood
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PHA-stimulated human lymphoctes (normal-resting-proliferating) at 0, 24, 48, 72, and 144 h were studied with Acridine Orange (AO) staining.
By viable cell sorting, by subsequent subculturing, and by use of biochemical, biophysical, and immunological assays, not only have the G0 resting and G1 (cycling) cell cycle phases been objectively characterized, but a separate subpopulation of quiescent cells that are functionally viable and deeply committed to nonproliferation, the Q cells, has been identified.
Multiparameter cytofluorimetric analysis, methyl14C-thymidine incorporation, automated image analysis, and mitogen stimulation studies have shown that the “Q” cell, compared to the “G0” resting but easily recruitable cell, exhibits quite lower red and green AO emission, possesses 2c to 4c DNA content (rather than only 2c), has a higher average optical density, and is either nonrecruitable or recruitable-with-difficulty in PHA-stimulated lymphocyte cultures.
Index EntriesReversible noncycling cells in blood nonreadily reversible cells in blood G0 cells in peripheral blood Q cells in peripheral blood blood, G0, and Q cells in human blood, G0 and Q cells in cell sorting, of G0, G1, and Q cells cytofluorometry of noncycling lymphocytes lymphocytes, characterization of automated image analysis, of human lymphocytes mitogen-stimulated lymphocytes, characterization of optical density, of human lymphocytes methyl14C-thymidine incorporation, in human lymphocytes
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