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Biological Trace Element Research

, Volume 71, Issue 1, pp 423–430 | Cite as

Limits of detection of a total reflection x-ray fluorescence system with double reflection module

  • V. F. Nascimento Filho
  • V. H. Poblete
  • P. S. Parreira
  • E. Matsumoto
  • S. M. Simabuco
  • E. P. Espinoza
  • A. A. Navarro
Section 5: Nuclear Analytical Methodology

Abstract

An X-ray tube with a Mo target and Zr filter, operated at 45 kV/20 mA, was used to excite samples (5 ΜL deposited on a quartz support) and the total reflection angle condition was obtained with a double reflector module built with two 10-cm-long 7-mm-thick quartz crystals placed 50 Μm apart. A high-resolution spectrometer based on a Si(Li) detector coupled to a multichannel analyzer was used for X-ray detection and the spectra were interpreted with the AXIL software.

The system was calibrated with standard chemical solutions containing Cr, Fe, Cu, Zn, and Pb, and Y was used as an internal standard to correct eventual geometric errors and high-voltage instabilities of the X-ray generator. The limits of detection were 19, 9, 5, and 4 ng/mL for Cr, Fe, Cu, and Zn, respectively, analyzed through characteristicKk α X-rays, and 7 ng/mL for Pb, throughLk α X-rays, considering 50 ΜL samples deposited and dried on a quartz support, to be excited/ detected for 1000 s.

Index Entries

Total reflection x-ray fluorescence TXRF XRF ED-XRF instrumental analysis 

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Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • V. F. Nascimento Filho
    • 1
    • 2
  • V. H. Poblete
    • 3
  • P. S. Parreira
    • 1
    • 4
  • E. Matsumoto
    • 5
  • S. M. Simabuco
    • 5
  • E. P. Espinoza
    • 1
  • A. A. Navarro
    • 1
  1. 1.Laboratŕio de InstrumentaÇÃo Muclear/CEMA/USPPiracicaba SÃo PauloBrazil
  2. 2.Departamento de Física e Meteorologia/ESALQ/USPPiracicaba, SÃo PauloBrazil
  3. 3.Laboratorio de Fluorescencia de Rayos X/CCHENSantiagoChile
  4. 4.Departamento de Física/CCE/UELCampus UniversitárioLondrina, ParanáBrazil
  5. 5.Departamento de Recursos Hidricos/FEC/UNICAMPCampus UniuersitárioCampinas, SÃo PauloBrazil

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